recD2
168
5'-3' DNA helicase replication fork progression, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation
locus
BSU_27480
Molecular weight
88.99 kDa
pI
5.08
function
replication fork progression, control of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] activity
product
5'-3' DNA helicase
essential
no
ec
3.6.4.12
synonyms
recD2, yrrC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0507 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,806,286 2,808,682
Phenotypes of a mutant
modest increase in spontaneous mutation rate [Pubmed|24443534]
[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] as well as [gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|search|ruvB ]combined mutants are not viable [pubmed|28527403]
The protein
Catalyzed reaction/ biological activity
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
[protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] polymerized on tailed SPP1 duplex intermediates invades a homologous region in another incomplete molecule, and in concert with [protein|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] helicase, reconstitutes a complete linear phage genome with redundant regions at the ends of the molecule [pubmed|31876108]
Protein family
RecD family (single member, according to UniProt)
Structure
[PDB|3E1S] (from Deinococcus radiodurans, 28% identity, from aa 178 - 767) [pubmed|18668125]
[AF|O34481]
Expression and Regulation
Operons
genes
[gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
description
[Pubmed|22383849]
additional information
protein has a low abundance (about 25 monomers per cell) [pubmed|35234892]
Biological materials
Mutant
MGNA-A858 (yrrC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/858 NBRP B. subtilis, Japan]
BKE27480 ([gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGCTGTCCCTCCTCCT, downstream forward: _UP4_TAAAATGCTCCCGTGCAAGC
BKK27480 ([gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGAGCTGTCCCTCCTCCT, downstream forward: _UP4_TAAAATGCTCCCGTGCAAGC
References
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Time of last update: 2025-01-18 17:04:16
Author of last update: Jstuelk