dgcW
168
diguanylate cyclase and potential phosphodiesterase
locus
BSU_13420
Molecular weight
84.63 kDa
pI
6.82
function
synthesis of c-di-GMP
product
diguanylate cyclase and potential phosphodiesterase
essential
no
synonyms
dgcW, ykoW
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3300 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,407,329 1,409,578
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-GMP from two molecules of GTP [Pubmed|23893111]
[wiki|Domains]
MHYT domain (aa 7-201) (according to UniProt)
contains a N-terminal [wiki|PAS domain] [Pubmed|23893111]
contains a central [wiki|GGDEF domain] and a C-terminal [wiki|EAL domain] [Pubmed|22821967]
aa 290-735 are similar to ''E. coli'' CsrD (18% identity, 43% similarity)
Structure
[PDB|5XGB] (from Pseudomonas aeruginosa, corresponds to the C-terminal part of DgcW, aa 194 ... 735, 29% identity) [pubmed|29109186]
[AF|O34311]
[wiki|Localization]
cell membrane (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A2E6852A5739B3C04405B98AFFC301A98E1ADCD0|dgcW]
description
[Pubmed|22383849]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
MGNA-B314 (ykoW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1313 NBRP B. subtilis, Japan]
BP140 (''[gene|A2E6852A5739B3C04405B98AFFC301A98E1ADCD0|dgcW]''::''ermC'') available in [wiki|Fabian Commichau]'s lab
BP142 (''[gene|A2E6852A5739B3C04405B98AFFC301A98E1ADCD0|dgcW]-[gene|B3E1652E9F3BDC9520847C479A2C0BED25025B4C|ykoV]-[gene|127CB0930A2D96B18D9261180028818F158C68CB|ligD]''::''kan'') available in [wiki|Fabian Commichau]'s lab
BKE13420 ([gene|A2E6852A5739B3C04405B98AFFC301A98E1ADCD0|dgcW]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT, downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT
BKK13420 ([gene|A2E6852A5739B3C04405B98AFFC301A98E1ADCD0|dgcW]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13420 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGGAGATTCCCCCCGT, downstream forward: _UP4_TAACAGCGCCGGCTTTTTTT
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-11-21 12:37:59
Author of last update: Jstuelk