ykuJ
168
unknown
locus
BSU_14100
Molecular weight
9.11 kDa
pI
4.34
function
unknown
product
unknown
essential
no
synonyms
ykuJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4703 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,484,117 1,484,356
The protein
Structure
[PDB|2FFG]
[AF|O34588]
Expression and Regulation
Operons
genes
[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]-[gene|FE15A41A58A8177280817CA3825764C39185021A|ccpC]
description
[Pubmed|16395550]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [Pubmed|14651647]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]~P [Pubmed|18840696,14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|16395550,12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
Biological materials
Mutant
MGNA-A771 (ykuJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/771 NBRP B. subtilis, Japan]
GP214 (deletion of ''[gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]-[gene|7FD784C885605F7FD27898BC9E83C28F21BE0E9D|ykuK]-[gene|899FF5BEE5D3F01778A0175E293EDBBDEB25717D|abbA]-[gene|90AFF93D6E7BC993B2773C12EE400C80A87A4831|darB]'', replaced by ''aphA3''), available in [wiki|Jörg Stülke]'s lab
BKE14100 ([gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT, downstream forward: _UP4_TAAGATTCCTGAAATAGGGG
BKK14100 ([gene|A26502ADAC214D45A8F7F394587B0BDFBE7AF28E|ykuJ]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTAAAAAAAGACTCCT, downstream forward: _UP4_TAAGATTCCTGAAATAGGGG
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP710 (in [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab, pGP714 (in [wiki|pAC6]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2025-01-29 18:49:52
Author of last update: Jstuelk