spoIVA
168
ATPase, spore coat morphogenetic protein, anchors the spore coat to the spore surface via [protein|BBDA32A4EE3389D6F4404F7B3DA9E13AC7BF8055|spoVM]
locus
BSU_22800
Molecular weight
55.01 kDa
pI
4.55
function
initiation of spore coat assembly
product
ATPase, basement layer protein for spore coat assembly
essential
no
synonyms
spoIVA, spoVP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5831 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,386,195 2,387,673
Phenotypes of a mutant
the spore coat does not localize to the spore surface but self-assembles into aggregates in the mother cell cytoplasm [Pubmed|22171814]
The protein
Catalyzed reaction/ biological activity
uses ATP hydrolysis to drive self-assembly into static filaments [Pubmed|23267091]
ATP hydrolysis drives polymerization of a nucleotide-free filament [Pubmed|23267091]
ploymerization depends on a critical threshold concentration of SpoIVA that is only achieved once the protein is recruited to the surface of the developing spore [Pubmed|23267091]
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
[wiki|Domains]
contains a Walker A ATPase domain
Structure
[AF|P35149]
Modification
phosphorylated on Arg-454 [pubmed|31221751]
[wiki|Localization]
innermost protein of the spore coat basement [Pubmed|22171814]
Expression and Regulation
Operons
genes
[gene|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA]
description
[Pubmed|1729246]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|1729246,15699190,15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|1729246,15699190], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE22800 ([gene|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC, downstream forward: _UP4_TAATACCGGTAGACCTCTTT
BKK22800 ([gene|A25C1530DA7BB007A288E525404E9F775E219FE8|spoIVA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGTGATCCCCTCCCGGAC, downstream forward: _UP4_TAATACCGGTAGACCTCTTT
References
Reviews
Original Publications
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Time of last update: 2024-12-19 11:24:21
Author of last update: Jstuelk