rapI
168
response regulator aspartate phosphatase, antagonist of [protein|DD1C8F3A4809785BD6A6047D39B42AB2C605E161|immR]
locus
BSU_05010
Molecular weight
46.40 kDa
pI
5.05
function
control of transfer of the mobile genetic element ICEBs1
product
response regulator aspartate phosphatase
essential
no
synonyms
rapI, yddL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
547,306 548,481
The protein
Catalyzed reaction/ biological activity
binds ImmR and inhibits its activity, this results in induction of the genes for the transfer of the mobile genetic element ICEBs1 [Pubmed|17511812]
Protein family
[wiki|RAP family] (according to UniProt)
[wiki|Domains]
six [wiki|TPR repeat|tetratrichopeptide repeats] (according to UniProt)
Structure
[PDB|4I1A] [Pubmed|23526881]
[AF|P96649]
Effectors of protein activity
binding of [protein|F3E14B772A5DA9073ED806A9ADDD9B64F0DFDD5B|phrI] indicates that neighbour cells do already contain the genetic element ICEBs1, this results in inactivation of RapI [Pubmed|17511812]
Expression and Regulation
Operons
genes
[gene|A208B4564A50D7BE2630DE40E8FAAF3C16236EE4|rapI]-[gene|F3E14B772A5DA9073ED806A9ADDD9B64F0DFDD5B|phrI]
description
[Pubmed|11466295]
regulation
induced at high cell density ([wiki|ComA]) [Pubmed|26582911]
regulatory mechanism
[protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]: activation, [Pubmed|26582911], in [regulon|protein:7832489F11F606BCF637EC23BAAB41AD10237EBB|comA regulon]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|11466295], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11466295], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE05010 ([gene|A208B4564A50D7BE2630DE40E8FAAF3C16236EE4|rapI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE05010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAAAATTCCCCCATCCT, downstream forward: _UP4_TTGATGAAAATCAGCCGGAT
BKK05010 ([gene|A208B4564A50D7BE2630DE40E8FAAF3C16236EE4|rapI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK05010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGAAAATTCCCCCATCCT, downstream forward: _UP4_TTGATGAAAATCAGCCGGAT
References
Reviews
Original Publications
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Time of last update: 2024-12-21 13:18:51
Author of last update: Jstuelk