uxaB
168
tagaturonate reductase
locus
BSU_12380
Molecular weight
55.19 kDa
pI
5.06
function
hexuronate utilization
product
tagaturonate reductase
essential
no
ec
1.1.1.58
synonyms
uxaB, yjmI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0246 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,309,880 1,311,322
The protein
Catalyzed reaction/ biological activity
D-altronate + NAD+ --> keto-D-tagaturonate + NADH (according to UniProt)
Protein family
mannitol dehydrogenase family (with [protein|4D54898D405EA25A0C74B1F02A492143D49B7B07|mtlD], according to UniProt)
Structure
[PDB|4IM7] (YdfI from E. coli, aa 1 - 370, 26% identity)
[AF|O34354]
Expression and Regulation
Operons
genes
[gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]
description
[Pubmed|9882655]
regulation
induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]
regulatory mechanism
[protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]: repression, in the absence of inducer, in [regulon|protein:69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882655], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,9882655], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
genes
[gene|5A0C29C7E218701727523885D01A48D02266B421|uxaC]-[gene|59CD3E4C24326542D9C85BB6FE64FBF49869EB55|yjmB]-[gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]
description
[Pubmed|9882655]
regulation
induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]
regulatory mechanism
[protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]: repression, in the absence of inducer, in [regulon|protein:69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882655], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A373 (yjmI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/373 NBRP B. subtilis, Japan]
BKE12380 ([gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCCTTATTGCCTCCCTCT, downstream forward: _UP4_TGCATACAAGGGGGAGAGGT
BKK12380 ([gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCCTTATTGCCTCCCTCT, downstream forward: _UP4_TGCATACAAGGGGGAGAGGT
References
Page visits: 2747
Time of last update: 2024-12-19 09:02:17
Author of last update: Melvin.boenninger