rph

rph
168

RNase PH, 3-5 exoribonuclease

locus
BSU_28370
Molecular weight
26.53 kDa
pI
4.89
Protein length
245 aaSequenceBlast
Gene length
738 bpSequenceBlast
function
3-5 exoribonuclease
product
RNase PH (EC 2.7.7.56)
essential
no
ec
2.7.7.56
synonyms
rph
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0689 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,901,154  2,901,891
The protein
Catalyzed reaction/ biological activity
major player in the exonucleolytic maturation of CCA-containing tRNA precursors [Pubmed|15983136]
responsible for endonucleolytic cleavage of scRNA (''[gene|097C817A5A3E31F73F6ABC4AA95852A64E43A057|scr]'') [Pubmed|17576666]
phosphate + tRNA(n+1) --> ribonucleoside 5'-diphosphate + tRNA(n) (according to UniProt)
Protein family
[wiki|RNase] PH family (single member, according to UniProt)
Structure
[PDB|1OYS]
[AF|P28619]
Expression and Regulation
Operons
genes
[gene|A18474CD50C2A1A83CF04C74098659BF1649B4DC|rph]-[gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]-[gene|13DF13F1EA21BC78035E5ED8B7AF2320235284A6|ysnB]
description
[Pubmed|22383849]
Open in new tab

[gene|A18474CD50C2A1A83CF04C74098659BF1649B4DC|rph]→[gene|13DF13F1EA21BC78035E5ED8B7AF2320235284A6|ysnB]

2024-12-22 06:00:59

Jstuelk

54

0f9e962c8f46d37722865610c6c0a69f06780cd3

B6C3C0619B9997D38F45FCD64F603B8C04463A79

Biological materials
Mutant
BKE28370 ([gene|A18474CD50C2A1A83CF04C74098659BF1649B4DC|rph]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTACCTCCGATTGT,  downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
BKK28370 ([gene|A18474CD50C2A1A83CF04C74098659BF1649B4DC|rph]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28370 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTACCTCCGATTGT,  downstream forward: _UP4_TAAAGACAGAAAGGCTTGAT
labs
[wiki|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
[wiki|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
31464530
Original Publications
17576666,15805522,19880604,14767080,1624460,15983136

A18474CD50C2A1A83CF04C74098659BF1649B4DC

Page visits: 2918

Time of last update: 2024-12-21 10:59:07

Author of last update: Jstuelk