tepA
168
orphan [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP]-like [wiki|germination] protease, contributes to SASP degradation
locus
BSU_16790
Molecular weight
24.10 kDa
pI
5.57
function
degradation of SASPs
product
[wiki|germination] protease
essential
no
synonyms
tepA, ymfB, ylxI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0740 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,751,201 1,751,938
The protein
Catalyzed reaction/ biological activity
degradation of SASPs in germinating spores [Pubmed|23927687]
Protein family
Peptidase S14 family (with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|clpP], according to UniProt)
Structure
[AF|Q99171]
Effectors of protein activity
activity requires [protein|A7C95C19ABD47456351C05A88B14F932BDEF1059|ylzJ] [Pubmed|23927687]
[wiki|Localization]
inner spore membrane [Pubmed|26731423]
Expression and Regulation
Operons
genes
[gene|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|tepA]-[gene|A7C95C19ABD47456351C05A88B14F932BDEF1059|ylzJ]
description
[Pubmed|22383849]
regulation
expressed late during [wiki|sporulation] in the forespore ([protein|search|SigG], [wiki|SpoVT]) [Pubmed|23123912]
regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|23123912], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|23123912], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-B377 (ymfB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1376 NBRP B. subtilis, Japan]
GP1120 (spc), available in [wiki|Jörg Stülke]'s lab
BKE16790 ([gene|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|tepA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCGCTTTCATCCTTTC, downstream forward: _UP4_AAAGAAGAAGGACGGATGAT
BKK16790 ([gene|A16C6515D185E3A268D57D77F28EF7EF9AF9FE05|tepA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCGCTTTCATCCTTTC, downstream forward: _UP4_AAAGAAGAAGGACGGATGAT
References
Reviews
Original Publications
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Time of last update: 2024-12-21 14:49:42
Author of last update: Jstuelk