yrbD
168
sodium/proton-dependent alanine transporter
locus
BSU_27810
Molecular weight
51.43 kDa
pI
9.38
function
uptake of alanine
product
sodium/proton-dependent alanine transporter
essential
no
synonyms
yrbD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1115 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,841,611 2,843,065
The protein
Protein family
[wiki|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)
Structure
[PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158]
[AF|O32060]
Paralogous protein(s)
[protein|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA], [protein|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT], [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT]
Expression and Regulation
Operons
genes
[gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]
description
[pubmed|22383849]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
Biological materials
Mutant
GP3556 (Δ[gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::phleo), available in [wiki|Jörg Stülke]'s lab
MGNA-A821 (yrbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/821 NBRP B. subtilis, Japan]
BKE27810 ([gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27810 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
BKK27810 ([gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
Expression vectors
pGP4013: expression of [gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD] (''gapA'' RBS) by [wiki|pBQ200] in ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
References
Page visits: 2840
Time of last update: 2024-12-29 04:05:36
Author of last update: Robert.warneke