uxuA
168
D-mannonate hydrolyase
locus
BSU_12340
Molecular weight
40.87 kDa
pI
4.92
function
hexuronate utilization
product
D-mannonate hydrolyase
essential
no
ec
4.2.1.8
synonyms
uxuA, yjmE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1312 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,305,486 1,306,565
The protein
Catalyzed reaction/ biological activity
D-mannonate --> 2-dehydro-3-deoxy-D-gluconate + H2O (according to UniProt)
Protein family
mannonate dehydratase family (single member, according to UniProt)
Structure
[PDB|1TZ9] (from Enterococcus faecalis, 44% identity)
[AF|O34346]
Expression and Regulation
Operons
genes
[gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]
description
[Pubmed|9882655]
regulation
induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]
regulatory mechanism
[protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]: repression, in the absence of inducer, in [regulon|protein:69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882655], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,9882655], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
genes
[gene|5A0C29C7E218701727523885D01A48D02266B421|uxaC]-[gene|59CD3E4C24326542D9C85BB6FE64FBF49869EB55|yjmB]-[gene|3AEA5D769B89E676C916FD8934D762B4C9A02AA2|yjmC]-[gene|CC55984E31C5698668CCCD4AC6E2210B2B41ACAB|yjmD]-[gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]-[gene|B886E504A767343042DE9BF4E23E6798D124A2CF|yjmF]-[gene|6D08E897DD697523FD0720704A25AD4472075B6C|exuT]-[gene|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]-[gene|A1C64447E0074B06C9210CC51680273FAAE703FA|uxaB]-[gene|ED29D2DE48AADEA09C90F08B6021555C357946B4|uxaA]
description
[Pubmed|9882655]
regulation
induced by galacturonate ([protein|search|ExuR]) [Pubmed|9882655]
regulatory mechanism
[protein|69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR]: repression, in the absence of inducer, in [regulon|protein:69B143987DCF73B5D080E3FD87A6CA2940E07DA8|exuR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|9882655] [pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882655], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A369 (yjmE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/369 NBRP B. subtilis, Japan]
BKE12340 ([gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCGCACCTCCGGATT, downstream forward: _UP4_ATGGCAAAAAAAGAGGTGGG
BKK12340 ([gene|A0A6D36BEC397345A1D17E6A7C34E1F612FD46C7|uxuA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCGCACCTCCGGATT, downstream forward: _UP4_ATGGCAAAAAAAGAGGTGGG
References
Page visits: 2953
Time of last update: 2024-12-20 00:59:24
Author of last update: Melvin.boenninger