yabA
168
inhibitor of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] oligomerization, tethers [protein|search|DnaA ]to the polymerase-clamp protein [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]
locus
BSU_00330
Molecular weight
13.96 kDa
pI
6.07
function
positioning of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]
product
negative regulator of [category|SW.3.1.1|replication] initiation
essential
no
synonyms
yabA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4467 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
42,499 42,858
Phenotypes of a mutant
strongly reduced growth rate in minimal medium [Pubmed|12060778]
over-initiation of [category|SW.3.1.1|replication] [pubmed|15927750]
a [gene|search|whiA ][gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] double mutant is not viable [pubmed|29378890]
synthetically lethal in combination with a [gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]1 mutation, this can be suppressed by mutations in [gene|CEE73284EFC0DBE8870CE0B474922DED79475A57|rel] or [gene|FD4E988BB73F6476CCEA4C521F343D6F8CBA457F|nrdR] that result in stimulated replication elongation, by mutations in [gene|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|dnaC] or [gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA] that result in decreased replication initiation as well as by inactivation of [gene|651F41AA16B0BB59A613D74302F441BF6D5BFF64|ccrZ] [pubmed|36053906]
The protein
Catalyzed reaction/ biological activity
negative control of replication initiation [Pubmed|12060778]
associates with the ''oriC'' region in a [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-dependent manner, this limits the amount of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] that can bind the ''oriC'' region [Pubmed|21895792]
YabA inhibits co-operative DNA binding by [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|21895792]
inhibits oligomerization and helix formation of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] [Pubmed|23909787]
Protein family
YabA family (single member, according to UniProt)
[wiki|Domains]
leucine zipper
[wiki|Cofactors]
Zinc [Pubmed|26615189]
Structure
[PDB|5DOL] [Pubmed|26615189]
[AF|P37542]
Modification
phosphorylated on Thr-71 by [protein|E86A96B832351FF513DD9853EAD8998CC44C9951|yabT] [pubmed|29619013]
Effectors of protein activity
[protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN] overexpression or release from the replisome decreases association of [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA] with ''oriC'', increases association of [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] with ''oriC'' [Pubmed|21895792]
[wiki|Localization]
forms foci in the midcell region, this depends on the interaction with [protein|6740108089F13116F200C15F35C2E7561E990FEB|dnaA] and [protein|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN] [Pubmed|26615189]
co-localizes with the origin region and replication forks throughout the cell cycle [pubmed|28166228]
Expression and Regulation
Operons
genes
[gene|08314FA6195D553B8522BCD3B45FEE0EAB51F64D|xpaC]-[gene|5231A0EE9C5A7556817B686D5B2B7B72B675BCAC|yaaN]-[gene|EEF3E572ED2F23581EB9B48D16B3112B886F7975|yaaO]-[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
genes
[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
regulation
expressed during growth and the transition phase, expression is erduced in stationary phase [Pubmed|23490197]
genes
[gene|5B9984BBCEC1244628DC1467BE26976AD8E06286|tmk]-[gene|31C8DCB4E1E225AC92EC2E11835234C4668AB326|darA]-[gene|3422E4C34EAAF355A9767F1AD8DFAE9A6A756F0C|yaaR]-[gene|DC867376EF4F9CC7663A9B76AA260AC3CA11595A|holB]-[gene|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT]-[gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]-[gene|55C4B4B7B46B6D04EF712BD79407C0B04DDC6A34|trmN6]-[gene|47859E62AABA5A57AC6F5F80CDE4ED52FC7F879F|yazA]-[gene|FB71FFDEABF17228CA2BD5F3DE0C8A9A2C22485D|yabC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B900 (yabA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1899 NBRP B. subtilis, Japan]
BKE00330 ([gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTATCCAAGGTTCCACACC, downstream forward: _UP4_TAGGGAAAAGGCTTCCGCAT
BKK00330 ([gene|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|yabA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTATCCAAGGTTCCACACC, downstream forward: _UP4_TAGGGAAAAGGCTTCCGCAT
labs
[wiki|Philippe Noirot], Jouy-en-Josas, France [http://locus.jouy.inra.fr/cms/index.php?id=18 Homepage]
[wiki|Alan Grossman], Cambridge, MA, USA [http://web.mit.edu/biology/www/facultyareas/facresearch/grossman.html Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 17:15:43
Author of last update: Jstuelk