thyB

thyB
168

thymidylate synthase B

locus
BSU_21820
Molecular weight
30.39 kDa
pI
6.27
Protein length
264 aaSequenceBlast
Gene length
795 bpSequenceBlast
function
biosynthesis of thymidine nucleotides
product
thymidylate synthase B
essential
no
ec
2.1.1.45
synonyms
thyB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0207 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,297,106  2,297,900
The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP --> 7,8-dihydrofolate + dTMP (according to UniProt)
Protein family
thymidylate synthase family (with [protein|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA], according to UniProt)
Structure
[PDB|3IX6] (from ''Brucella melitensis'', 67% identity)
[AF|P11044]
Modification
phosphorylated on Arg-102 [Pubmed|22517742]
Paralogous protein(s)
[protein|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA]
Expression and Regulation
Operons
genes
[gene|8B515C789439354F870023E69077D2C1D709E046|ypjP]-[gene|2CB3BAF17F59A858D249619A436EF660CAA58DD5|ypjQ]-[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]-[gene|9D1DAA5E69DD12FDE0FB33EA0863E0CB214120EC|ypkP]
description
[Pubmed|22383849]
Open in new tab

[gene|8B515C789439354F870023E69077D2C1D709E046|ypjP]→[gene|9D1DAA5E69DD12FDE0FB33EA0863E0CB214120EC|ypkP]

2024-12-13 18:21:57

Jstuelk

96

235b0d972d347f04b87f4c45ebac71a8a9d4b96a

9EB04D4A3A7698B00A36018A9A11CB02D30C8250

genes
[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]
description
[Pubmed|2840350]
regulation
weak induction in the presence of glucose ([protein|search|CcpN]) [Pubmed|19732150]
regulatory mechanism
[protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]: activation, weak, in [regulon|protein:2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN regulon]
Open in new tab

[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]→[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]

2024-12-21 14:37:22

ghost

76

c85a29ba6a879af8f10ee961ab515c0e0a8b2658

B9BDE226BFA3A6A21A7E3540C80A424429DFA9B3

Biological materials
Mutant
BKE21820 ([gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA,  downstream forward: _UP4_GTATGATTTCATTCATTTTT
BKK21820 ([gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21820 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA,  downstream forward: _UP4_GTATGATTTCATTCATTTTT
References
Reviews
7574499
Original Publications
418407,19732150,2840350,22517742,29150519

9F9643D51E9ABA537892B19312491697CECDDCCF

Page visits: 2945

Time of last update: 2024-12-21 18:06:58

Author of last update: Melvin.boenninger