thyB
168
thymidylate synthase B
locus
BSU_21820
Molecular weight
30.39 kDa
pI
6.27
function
biosynthesis of thymidine nucleotides
product
thymidylate synthase B
essential
no
ec
2.1.1.45
synonyms
thyB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0207 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,297,106 2,297,900
The protein
Catalyzed reaction/ biological activity
(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP --> 7,8-dihydrofolate + dTMP (according to UniProt)
Protein family
thymidylate synthase family (with [protein|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA], according to UniProt)
Structure
[PDB|3IX6] (from ''Brucella melitensis'', 67% identity)
[AF|P11044]
Modification
phosphorylated on Arg-102 [Pubmed|22517742]
Paralogous protein(s)
[protein|6FE1D3C4D4DF86DE2D3D9A143A1DCFE120F13F5F|thyA]
Expression and Regulation
Operons
genes
[gene|8B515C789439354F870023E69077D2C1D709E046|ypjP]-[gene|2CB3BAF17F59A858D249619A436EF660CAA58DD5|ypjQ]-[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]-[gene|9D1DAA5E69DD12FDE0FB33EA0863E0CB214120EC|ypkP]
description
[Pubmed|22383849]
genes
[gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]-[gene|B740E971204AC14BA456B8C93A86D4BDC29524FF|dfrA]
description
[Pubmed|2840350]
regulation
weak induction in the presence of glucose ([protein|search|CcpN]) [Pubmed|19732150]
regulatory mechanism
[protein|2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN]: activation, weak, in [regulon|protein:2234D81F8F9B92EAF1CF6F73BEC92D4DE54E2636|ccpN regulon]
Biological materials
Mutant
BKE21820 ([gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA, downstream forward: _UP4_GTATGATTTCATTCATTTTT
BKK21820 ([gene|9F9643D51E9ABA537892B19312491697CECDDCCF|thyB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTTTCATCCTTTAAA, downstream forward: _UP4_GTATGATTTCATTCATTTTT
References
Reviews
Original Publications
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Time of last update: 2024-10-07 17:59:42
Author of last update: Melvin.boenninger