ynbA

ynbA
168

GTP-binding protein, ribosome-splitting factor, rescues translationally arrested ribosomes

locus
BSU_17430
Molecular weight
37.51 kDa
pI
9.24
Protein length
Gene length
function
rescuing of translationally arrested ribosomes
product
GTP-binding protein, ribosome-splitting factor
essential
no
synonyms
ynbA, hflX

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2262 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,875,304  1,876,566
The protein
Catalyzed reaction/ biological activity
binds and hydrolyzes GTP and readily exchanges GDP for GTP
rescuing of translationally arrested ribosomes [Pubmed|26458047]
Protein family
TRAFAC class OBG-HflX-like GTPase superfamily (with [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg] and [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], according to UniProt)
[wiki|Domains]
Hflx-type G domain (aa 201-363) (according to UniProt)
Structure
[PDB|5ADY] (the protein from ''E. coli'' in complex with the [wiki|ribosome], 41% identity) [Pubmed|26458047]
[AF|P94478]
Effectors of protein activity
binds ppGpp [Pubmed|26951678], ppGpp may inhibit activity [Pubmed|26951678]
Paralogous protein(s)
[protein|B2270316642C66BC2DA86EBDCD1BF6A33CC6A1DC|engA], [protein|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF], [protein|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era], [protein|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC], [protein|54CD247926F7FABE4ACE85EB7021B62500B47260|rbgA], [protein|66A6EC4F6CB91EE741BB9D7646913FF3EE602BD7|thdF], [protein|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg], [protein|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]
Expression and Regulation
Operons
genes
[gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]-[gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB]-[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]
description
[Pubmed|22383849]
regulation
the mRNA is processed between [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] and [gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
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[gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]→[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]

2024-12-08 14:01:46

Jstuelk

208

d45811f6bc7bef80a041d78aaa209f36dccdee6a

2408FECBCAE7255480B230470AF58DDA9F084F5A

Biological materials
Mutant
MGNA-A020 (ynbA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/20 NBRP B. subtilis, Japan]
BKE17430 ([gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT,  downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
BKK17430 ([gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATGGATGTTCCTTTCTT,  downstream forward: _UP4_ATGTAGGAAGGAAATATAAA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Naotake Ogasawara], Nara, Japan
References
Reviews
21885683
Original Publications
12427945,26458047,26951678,26951678

9F6B78C1932FB56F7F71430DB16BC862A312407B

Page visits: 2796

Time of last update: 2024-12-08 13:46:57

Author of last update: Robert.warneke