bkdAA
168
2-oxoisovalerate dehydrogenase (E1 alpha subunit)
locus
BSU_24050
Molecular weight
36.18 kDa
pI
4.78
function
utilization of branched-chain keto acids
product
2-oxoisovalerate dehydrogenase (E1 alpha subunit)
essential
no
ec
1.2.4.4
synonyms
bkdAA, bfmBAA, bfmB1a, bkd
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,499,090 2,500,082
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
a strain lacking the [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] operon and the [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des] gene exhibits strongly reduced membrane fluidity and eventual growth arrest [pubmed|35037270]
The protein
Catalyzed reaction/ biological activity
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-lipoyl-L-lysine + H+ --> [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase]-(R)-N6-(S8-2-methylpropanoyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)
Protein family
BCKDHA family (single member, according to UniProt)
[wiki|Cofactors]
TPP [pubmed|35988322]
Structure
[PDB|1UMB] (from '' Thermus thermophilus'', 42% identity, 57% similarity) [Pubmed|15033367]
[AF|P37940]
Paralogous protein(s)
[protein|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA], [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]
[wiki|Localization]
Membrane-proximal (Spotty) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]
regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]: activation, [Pubmed|10094682], in [regulon|protein:4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|10094682], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|10094682], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
Biological materials
Mutant
BKE24050 ([gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAG
BKK24050 ([gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAAGCCCCTCCTTTAT, downstream forward: _UP4_TATGCGAAGTAGGGAGGAAG
References
Reviews
Original Publications
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Time of last update: 2024-11-06 04:02:00
Author of last update: Jstuelk