hypO
168
NADPH-flavin nitroreductase
locus
BSU_07830
Molecular weight
25.48 kDa
pI
5.67
function
protection against NaOCl stress
product
NADPH-flavin nitroreductase
essential
no
synonyms
hypO, yfkO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
854,412 855,077
Phenotypes of a mutant
a ''[gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO] [gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]'' mutant is more sensitive to NaOCl stress than ''[gene|869E46AECF6249610F8541E403D834284B62171D|ohrA]'' single mutant [Pubmed|22238377]
The protein
Catalyzed reaction/ biological activity
reduction of 2,6-dinitrotoluene [pubmed|31392514]
Protein family
[wiki|nitroreductase family] (according to UniProt)
[wiki|Cofactors]
[metabolite|FMN] [Pubmed|21635694] (according to UniProt)
[metabolite|NADP] [pubmed|31392514]
Structure
[PDB|8QYG] (from Bacillus tequilensis, 97.8% identity) [pubmed|38946302]
[AF|O34475]
Expression and Regulation
Operons
genes
[gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO]
description
[Pubmed|22238377]
regulation
induced under disulfide stress conditions (diamide, NaOCl) ([protein|search|HypR]) [Pubmed|22238377]
regulatory mechanism
[protein|91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR]: activation, [Pubmed|22238377], in [regulon|protein:91085DA5E71B88E533D2E1D1BFB53E908116C6AA|hypR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22238377], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-C366 (yfkO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2364 NBRP B. subtilis, Japan]
BKE07830 ([gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACACCTCTCCTT, downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
BKK07830 ([gene|9DF5999568760E016ECA988385A1FA61A257D486|hypO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTAAAACACCTCTCCTT, downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
labs
[wiki|Haike Antelmann], University of Greifswald, Germany
References
Original Publications
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Time of last update: 2025-01-03 07:01:25
Author of last update: Jstuelk