ganB
168
endo-1,4-beta-galactosidase
locus
BSU_34120
Molecular weight
47.12 kDa
pI
8.24
function
galactan utilization
product
endo-1,4-beta-galactosidase
essential
no
ec
3.2.1.89
synonyms
ganB, yvfO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3867 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,501,651 3,502,940
Phenotypes of a mutant
a ''[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]'' mutant is impaired in the utilization of galactan [Pubmed|22893383]
The protein
Catalyzed reaction/ biological activity
Endohydrolysis of (14)-beta-D-galactosidic linkages in galactan, generation of galactotriose [Pubmed|22893383]
Protein family
glycosyl hydrolase 53 family (single member, according to UniProt)
Structure
[PDB|2J74] (B. licheniformis)
[AF|O07013]
[wiki|Localization]
likely to be extracellular, carries a predicted a signal peptide of 26 amino acid residues at the N-terminus [Pubmed|27501980,22893383]
Expression and Regulation
Operons
genes
[gene|D4A5A878F87EBC14C02824233155CEBACEB41343|ganS]-[gene|75B1FD4AA7612B3B600CB0CE35319A6960536095|ganP]-[gene|676932E7DF143DC9AAAF1F415FB3D14EE25B2C2F|ganQ]-[gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA]-[gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]
description
[Pubmed|9287030]
regulation
induced by galactan (binding of galactobiose to [protein|1F787F70C87DEB221709579122D63C2322A84E56|ganR]) [Pubmed|28617843,27501980]
repressed by glucose ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|28617843,27501980]
regulatory mechanism
[protein|1F787F70C87DEB221709579122D63C2322A84E56|ganR]: repression, [Pubmed|9287030], in [regulon|protein:1F787F70C87DEB221709579122D63C2322A84E56|ganR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|28617843], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|27501980], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A487 (yvfO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/487 NBRP B. subtilis, Japan]
BKE34120 ([gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCATCCCCCCAAG, downstream forward: _UP4_TGAAACAAAAAAATCCATGA
BKK34120 ([gene|9DDEF5682B2D145830F33C7A1D5A6EC8527BB405|ganB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTTCATCCCCCCAAG, downstream forward: _UP4_TGAAACAAAAAAATCCATGA
References
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Time of last update: 2025-04-14 22:58:27
Author of last update: Melvin.boenninger