spoIIIAE
168
component of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] type III secretion system residing in the forespore membrane, required for [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activation
locus
BSU_24390
Molecular weight
43.68 kDa
pI
6.59
function
activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]
product
component of a type III secretion system
essential
no
synonyms
spoIIIAE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5826 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,534,326 2,535,525
The protein
Structure
[AF|P49782]
[wiki|Localization]
membrane protein [Pubmed|18485064], insertion into the membrane requires [protein|D40C202E67A5319A91811DB11356F47A56C97DD2|yidC1] [Pubmed|18485064]
Expression and Regulation
Operons
genes
[gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]-[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|1766372]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,18485064], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
genes
[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|18485064]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
Biological materials
Mutant
BKE24390 ([gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTCAATCTACCGCCTCCTT, downstream forward: _UP4_ATGATGAAATGAAGGAGGCA
BKK24390 ([gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTCAATCTACCGCCTCCTT, downstream forward: _UP4_ATGATGAAATGAAGGAGGCA
labs
[wiki|Richard Losick], Harvard University, Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
[wiki|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]
References
Reviews
Original Publications
Page visits: 2192
Time of last update: 2024-10-13 07:42:48
Author of last update: Jstuelk