yetO
168
cytochrome P450 (CYP102A2)/ NADPH-cytochrome P450 reductase
locus
BSU_07250
Molecular weight
119.26 kDa
pI
5.73
function
fatty acid metabolism
product
NADPH-cytochrome P450 reductase
essential
no
ec
1.6.2.4
synonyms
yetO, yfnJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
792,682 795,867
The protein
Catalyzed reaction/ biological activity
oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids [Pubmed|15375636]
alkane + O2 + reduced [NADPH—hemoprotein reductase] --> primary alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
NADPH + 2 oxidized [cytochrome P450] --> H+ + NADP+ + 2 reduced [cytochrome P450] (according to UniProt)
Protein family
[wiki|cytochrome P450] family (according to UniProt)
[wiki|Domains]
[wiki|Flavodoxin-like domain] (aa 493-632) (according to UniProt)
[wiki|FAD-binding FR-type domain] (aa 671-904) (according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|2X7Y] (from ''Bacillus megaterium''; 66% identity, 87% similarity) [Pubmed|25325618,22949185]
[AF|O08394]
Paralogous protein(s)
[protein|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C225 (yfnJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2223 NBRP B. subtilis, Japan]
BKE07250 ([gene|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGAACCATTCCTTTCCG, downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
BKK07250 ([gene|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|yetO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGAACCATTCCTTTCCG, downstream forward: _UP4_TAGATAAAGAAGACTGGAGA
References
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Time of last update: 2024-12-26 08:31:34
Author of last update: Melvin.boenninger