khtU
168
K+/H+ antiporter for K+ efflux
locus
BSU_09850
Molecular weight
43.79 kDa
pI
9.29
function
potassium ion efflux
product
K+/H+ antiporter for K+ efflux
essential
no
synonyms
khtU, yhaU
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0475 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,059,203 1,060,420
Phenotypes of a mutant
increased sensitivity to methylglyoxal [Pubmed|24330391]
The protein
Protein family
Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family (with [protein|6985ED02AC7A04D4FD5E531C47DD4D1711D7F829|cpaA], according to UniProt)
Structure
[PDB|4BWZ] (sodium proton antiporter NapA from Thermus thermophilus, 26% identity) [pubmed|23995679]
[AF|O07536]
Effectors of protein activity
potassium export is stimulated by binding of c-di-AMP to [protein|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT] [pubmed|33790011]
[wiki|Localization]
cell membrane, integral membrane protein (according to UniProt)
Expression and Regulation
Operons
genes
[gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-A682 (yhaU::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/682 NBRP B. subtilis, Japan]
GP2078 ([gene|A54E8E38AEF596D09B4BEB64D9F539A8AD34E73D|khtS]-[gene|D8A8FBC05B9665F72A88685D2B33822C2BB84C62|khtT]-[gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::''aphA3''), available in [wiki|Jörg Stülke]'s lab
BKE09850 ([gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
BKK09850 ([gene|9A54C82323BA9220CAA6041E3745FAB9E222FE78|khtU]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAATACAAGATGGTCCATCG, downstream forward: _UP4_GGATAAAAAAAGCAGCACTG
FLAG-tag construct
GP2432 ''khtU-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-21 02:16:48
Author of last update: Jstuelk