liaS
168
two-component sensor kinase/phosphatase, response to bacitracin
locus
BSU_33090
Molecular weight
40.55 kDa
pI
7.33
function
control of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR] activity in response to bacitracin
product
two-component sensor kinase
essential
no
synonyms
liaS, yvqE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,395,035 3,396,117
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
dephosphorylation of [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR] in the absence of the stress signal [Pubmed|23279150]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments
[wiki|HAMP domain] (aa 74-126) (according to UniProt)
[wiki|Histidine kinase domain] (aa 153-346) (according to UniProt)
Structure
[PDB|3GIG] ([protein|C5F9A1970393625B01C05C72C9A240C8EE8789D3|desK], corresponds to the C-terminal domain, aa 149 ... 343, 25% identity) [pubmed|19805278]
[AF|O32198]
Modification
autophosphorylation on a His residue
Effectors of protein activity
LiaS kinase activity is inhibited by [protein|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF], phosphatase acitivity is maintained by [protein|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF] in the absence of the stress signal [Pubmed|23279150]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
description
[Pubmed|16816187]
regulation
''[protein|search|liaG]'': constitutive
regulatory mechanism
[protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]: activation, [Pubmed|16816187], in [regulon|protein:49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15273097], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]
description
[Pubmed|14651641]
regulation
''[protein|search|liaG]'': constitutive
Biological materials
Mutant
MGNA-B036 (yvqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1035 NBRP B. subtilis, Japan]
BKE33090 ([gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
BKK33090 ([gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GAGGCTGGCAAGCATTTTTT, downstream forward: _UP4_CCGGAAGAAAAAGGAGAGAA
labs
[wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
References
Reviews
Original Publications
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Time of last update: 2024-10-15 05:22:01
Author of last update: Jstuelk