thiD
168
4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase
locus
BSU_11710
Molecular weight
28.97 kDa
pI
5.71
function
biosynthesis of thiamine pyrophosphate (TPP)
product
4-amino-5-hydroxymethyl-2-methylpyrimidinepyrophosphate kinase
essential
no
ec
2.7.1.49
synonyms
thiD, yjbV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0351 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,246,837 1,247,652
The protein
Catalyzed reaction/ biological activity
4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP --> 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H+ (according to UniProt)
4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP --> 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP (according to UniProt)
Protein family
ThiD family (with [protein|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK], according to UniProt)
Structure
[PDB|2I5B] [Pubmed|16978644]
[AF|O31620]
Paralogous protein(s)
[protein|FEDD589EA9982856730E809F21B7930F688FDC42|pdxK]
Expression and Regulation
Operons
genes
[gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]
description
[Pubmed|1898926]
regulation
repressed by thiamine ([wiki|Thi-box]) [Pubmed|16356850]
antitermination is modulated by [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|nusG]-dependent transcription pausing just upstream of the [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box] [pubmed|39494516]
the [wiki|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
[protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]
Biological materials
Mutant
MGNA-B167 (yjbV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1166 NBRP B. subtilis, Japan]
BKE11710 ([gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTCATTACACTACACTCCT, downstream forward: _UP4_TGAGATCACCAGCTGATGGT
BKK11710 ([gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTCATTACACTACACTCCT, downstream forward: _UP4_TGAGATCACCAGCTGATGGT
References
Reviews
Original Publications
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Time of last update: 2025-04-02 02:15:23
Author of last update: Melvin.boenninger