fruA
168
fructose-specific permease of the [wiki|phosphotransferase systems|phosphotransferase system], EIIABC of the [category|SW.1.2.2|PTS]
locus
BSU_14400
Molecular weight
67.01 kDa
pI
5.24
function
fructose uptake and phosphorylation
product
fructose-specific [category|SW.1.2.2|PTS], EIIABC
essential
no
ec
2.7.1.69
synonyms
fruA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1762 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,509,256 1,511,163
The protein
Catalyzed reaction/ biological activity
D-fructose + Nπ-phospho-L-histidyl-[protein] --> D-fructose 1-phosphate + L-histidyl-[protein] (according to UniProt)
Protein family
[category|SW.1.2.2|PTS] permease, fructose/ mannitol family [Pubmed|10627040]
[wiki|Domains]
[wiki|PTS EIIA domain] type-2 (aa 5-149) (according to UniProt)
[wiki|PTS EIIB domain] type-2 (aa 172-267) (according to UniProt)
[wiki|PTS EIIC domain] type-2 (aa 301-635) (according to UniProt)
Structure
[PDB|2R4Q] (EIIB domain)
[AF|P71012]
Paralogous protein(s)
[protein|575CEC5C5C0458DC86A74F24654AC6990CB1E732|manP]
[wiki|Localization]
cell membrane (homogeneous) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|fruR]-[gene|502AA809C8C95F8886DD91FBB7DDBECC63342327|fruK]-[gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]
description
[pubmed|22383849]
regulation
induced in the presence of fructose ([protein|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|fruR]) (according to [http://dbtbs.hgc.jp/COG/prom/fruRKA.html DBTBS])
regulatory mechanism
[protein|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|fruR]: repression, (according to [http://dbtbs.hgc.jp/COG/prom/fruRKA.html DBTBS]), in [regulon|protein:7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|fruR regulon]
Biological materials
Mutant
BKE14400 ([gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
BKK14400 ([gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATTTTCACTTGTGTTTCCT, downstream forward: _UP4_TAAGAAAAAAAGCGTCCTTG
References
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Time of last update: 2024-12-22 12:03:39
Author of last update: Melvin.boenninger