thiT
168
S protein of thiamine [wiki|ECF transporter]
locus
BSU_30990
Molecular weight
20.31 kDa
pI
10.24
function
thiamine uptake
product
S protein of thiamine [wiki|ECF transporter]
essential
no
synonyms
thiT, yuaJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3859 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,179,306 3,179,884
The protein
Protein family
vitamin uptake transporter (VUT/ECF) (TC 2.A.88) family (with [protein|97573C2D4FD8BE6B4C749C0644D053C9A0CBFFF3|trpP] and [protein|7DD4BB8222B2EFE81C32C5521A29256095B9D6C1|ypdP], according to UniProt)
Structure
[PDB|3RLB] (from ''Lactococcus lactis'', 32% identity) [Pubmed|21706007]
[AF|O32074]
[wiki|Localization]
membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]
description
[pubmed|22383849]
regulation
repressed by thiamine ([regulon|Thi-box|]) [Pubmed|12376536]
regulatory mechanism
[protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, [pubmed|], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]
Biological materials
Mutant
GP4787 (trpC2 Δ[gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]::neo), available in [wiki|Jörg Stülke]'s lab
MGNA-A222 (yuaJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/222 NBRP B. subtilis, Japan]
BKE30990 ([gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE30990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATCTCCTTCCATT, downstream forward: _UP4_TAAAAGTAACAATCCCCCAG
BKK30990 ([gene|9685A380F95F1A73A693B53E4D35A1CB51043FEC|thiT]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK30990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCTATCTCCTTCCATT, downstream forward: _UP4_TAAAAGTAACAATCCCCCAG
References
Reviews
Original Publications
Page visits: 3729
Time of last update: 2025-01-28 10:52:04
Author of last update: Robert.warneke