dhbF
168
involved in 2,3-dihydroxybenzoate biosynthesis
locus
BSU_31960
Molecular weight
263.44 kDa
pI
4.74
function
biosynthesis of the siderophore bacillibactin
product
unknown
essential
no
synonyms
dhbF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3319 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,280,519 3,287,655
The protein
Protein family
[wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)
[wiki|Domains]
2 [wiki|Carrier domain]s (aa 961-1036, aa 2036-2111) (according to UniProt)
Structure
[PDB|2VSQ] (termination module of [protein|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC], 31% identity) [pubmed|18583577]
[AF|P45745]
Modification
phosphorylation on Ser-988 AND Ser-996 [Pubmed|17218307]
[wiki|Localization]
membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
description
[Pubmed|8921902]
regulation
induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
expression is increased in the presence of pulchimerrin [pubmed|37137890]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8550523], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
additional information
the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
Biological materials
Mutant
MGNA-A063 (yukL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/63 NBRP B. subtilis, Japan]
BKE31960 ([gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC, downstream forward: _UP4_AATAAATAAGGGAGGAGAAA
BKK31960 ([gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCGTTTGTTTACCCTCC, downstream forward: _UP4_AATAAATAAGGGAGGAGAAA
References
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Time of last update: 2024-12-31 00:45:17
Author of last update: Melvin.boenninger