spoVAD
168
essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores and DPA release during [category|SW.4.2.4|Germination], likely germination protein
locus
BSU_23410
Molecular weight
35.84 kDa
pI
5.24
function
likely germination protein
product
binding protein for DPA
essential
no
synonyms
spoVAD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5837 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,440,775 2,441,791
The protein
Catalyzed reaction/ biological activity
maybe involved in spore [wiki|germination] [Pubmed|22327596]
binding of both DPA(2,6) and Ca(2 )-DPA(2,6) [Pubmed|22328679]
forms a membrane complex with [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC] and [protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] in vitro [pubmed|35654455]
functions like a "plug" for the [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]/[protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] membrane channel [pubmed|35654455]
Structure
[PDB|3LM6]
[AF|P40869]
[wiki|Localization]
cytoplasmic side of the spore inner membrane - that depends on [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]/[protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] [Pubmed|35654455,23335419,16077113]
Additional information
6,500 molecules are present per spore [Pubmed|23749970]
Expression and Regulation
Operons
genes
[gene|5410018998EC9792A69CF0D9C438EEF5AC8A82C5|spoVAA]-[gene|AEC70413BDC29F435776D532E7049AA9A0BD893D|spoVAB]-[gene|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]-[gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]-[gene|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB]-[gene|B44529B69769D7AF1010E06C95E398EA91A3E798|spoVAEA]-[gene|CBE5093DFBBCE25071CCE12408BC9AFF7A158C03|spoVAF]-[gene|02F1C926D200DE85C93A0ACEF23AD25FBD12B0BD|lysA]
description
[Pubmed|7934830]
regulation
expressed late during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], [wiki|SpoVT]) [Pubmed|15699190,1903432,8755877]
regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: activation, [Pubmed|8755877], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190,1903432], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
additional information
6,500 molecules are present per spore [PubMed|23749970]
Biological materials
Mutant
BKE23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
BKK23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
labs
[wiki|Peter Setlow], University of Connecticut Health Center, USA
References
Reviews
Original Publications
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Time of last update: 2024-12-29 21:58:41
Author of last update: Jstuelk