argG
168
argininosuccinate synthase, reversible
locus
BSU_29450
Molecular weight
44.66 kDa
pI
5.03
function
biosynthesis of arginine
product
argininosuccinate synthase, reversible
essential
no
ec
6.3.4.5
synonyms
argG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0137 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,013,133 3,014,344
The protein
Catalyzed reaction/ biological activity
ATP + L-aspartate + L-citrulline --> 2-(N-L-arginino)succinate + AMP + diphosphate + H+ (according to UniProt)
Protein family
argininosuccinate synthase family (single member, according to UniProt)
Structure
[PDB|1KH1] (from ''Thermus thermophilus'', 44% identity, 65% similarity) [Pubmed|11844799]
[AF|O34347]
Modification
S-cysteinylation after diamide stress (C187) [Pubmed|17611193]
phosphorylated on Arg-262 [Pubmed|22517742]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]-[gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH]
description
[Pubmed|22383849]
regulation
repressed by arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC])
induced in the absence of [protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM] [pubmed|30355672]
regulatory mechanism
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: repression, in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]: unknown, [pubmed|30355672], in [regulon|protein:F4097349A563503468A2A14F062AEAC532C7917A|rnpM regulon]
Biological materials
Mutant
GP1688 ([gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]-[gene|571789ADA1F7B9DE0F9E205E2DEB9C9166F6C312|argH])::''aphA3'', available in [wiki|Jörg Stülke]'s lab
GP3711 ([gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]::aphA3 trpC2) available in the [wiki|Stülke] lab
GP3984 ([gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::PargGH-lacZ-aphA3) suitable for quantification of expression of the argGH operon, available in [wiki|Jörg Stülke]'s lab
BKE29450 ([gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
BKK29450 ([gene|94E5DA4F460FBC02A186D1013C581179F13BCB3E|argG]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAATCCCCTCTC, downstream forward: _UP4_GCATGAAGAAGCTTTGGGGA
Expression vectors
pGP3766: expression of Strep-''argG'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP3778: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP3984 (aphA3, based on [wiki|pAC7]]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-21 10:07:16
Author of last update: Robert.warneke