asnH
168
asparagine synthetase (glutamine-hydrolysing)
locus
BSU_39920
Molecular weight
85.66 kDa
pI
5.53
function
biosynthesis of asparagine
product
asparagine synthetase (glutamine-hydrolysing)
essential
no
ec
6.3.5.4
synonyms
asnH, yxaN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,098,926 4,101,169
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)
Protein family
asparagine synthetase family (with [protein|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB] and [protein|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO], according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-2 domain] (aa 2-218) (according to UniProt)
Structure
[PDB|1CT9] (from E. coli, corresponds to aa 30 ... 537, 27% identity) [pubmed|10587437]
[AF|P42113]
Expression and Regulation
Operons
genes
[gene|787BB72119DB1321A8281D7C46FED28CAEDBC97C|yxbB]-[gene|88B01CC87394AEE693A98574F9343EBF99F0D60E|yxbA]-[gene|A15368CD02487EBC1AF999FED34672E38167BCB2|yxnB]-[gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]-[gene|BF0E188249161B3CC24D223FDD48B820EBED8F55|yxaM]
description
[Pubmed|10746760]
regulation
repressed by glucose (8-fold) [Pubmed|12850135]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|15101989], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|20185509], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
GP4146 (''asnH''::''ermC''), available in [wiki|Jörg Stülke]'s lab
MGNA-B689 (asnH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1688 NBRP B. subtilis, Japan]
BKE39920 ([gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTCTCCCTCCCAATA, downstream forward: _UP4_AATCTGCCAGAAGGAGCATA
BKK39920 ([gene|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTCTCCCTCCCAATA, downstream forward: _UP4_AATCTGCCAGAAGGAGCATA
References
Reviews
Original Publications
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Time of last update: 2025-04-05 01:13:35
Author of last update: Jstuelk