dgkB
168
diacylglycerol kinase
locus
BSU_06720
Molecular weight
33.18 kDa
pI
5.64
function
biosynthesis of lipoteichoic acid
product
diacylglycerol kinase
essential
no
ec
2.7.1.107
synonyms
dgkB, yerQ, dagK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1597 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
736,436 737,347
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
essential [Pubmed|12682299], non-essential according to [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycerol + ATP --> 1,2-diacyl-sn-glycero-3-phosphate + ADP + H+ (according to UniProt)
Protein family
diacylglycerol/lipid kinase family (with [protein|88AECCE90DC0D6C92C61AB8860A31A830A8DAC42|bmrU] and [protein|ED4C1EA16FF96DA20F12B972CD2458FA3B49DDA5|ytlR], according to UniProt)
[wiki|Domains]
DAGKc domain (aa 1-132) (according to UniProt)
Structure
[PDB|3S40] (from ''B. anthracis'', 32% identity, 68% similarity)
[AF|O31502]
Paralogous protein(s)
[protein|88AECCE90DC0D6C92C61AB8860A31A830A8DAC42|bmrU]
Expression and Regulation
Operons
genes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]
description
[Pubmed|22383849]
genes
[gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]-[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]-[gene|5B5014A68D777DB79179904D5FD3D14DEE0BF1F4|yefC]-[gene|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-[gene|2E55D086DD5023B98929FB8B61238C00E66B8BCB|yeeB]-[gene|DB1AAC91947A6008F631266E5CC85B58703AFD9D|yeeC]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B448 (yerQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1447 NBRP B. subtilis, Japan]
GP1391 ''dgkB::cat ltaS::aphA3'', available in [wiki|Jörg Stülke]'s lab
BKE06720 ([gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCTCATCATCCTACT, downstream forward: _UP4_TAAAACTTGGCTTGGTAAGC
BKK06720 ([gene|943294ACA74067A8549056699E08DF5B82337976|dgkB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATCTCATCATCCTACT, downstream forward: _UP4_TAAAACTTGGCTTGGTAAGC
References
Reviews
Original Publications
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Time of last update: 2024-10-05 19:35:55
Author of last update: Jstuelk