csrA
168
motility regulator, binds to the [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag] mRNA to inhibit its translation, promotes the base pairing between the [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA and the [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] mRNA
locus
BSU_35370
Molecular weight
8.00 kDa
pI
6.09
function
control of [gene|search|hag ]translation
product
RNA chaperone, motility regulator
essential
no
synonyms
csrA, yviG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1551 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,636,046 3,636,270
The protein
Catalyzed reaction/ biological activity
[protein|93F328524989597C7D2329B25E665496C9631E87|csrA] binds the [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag] mRNA to inhibit translation [Pubmed|21895793]
binds [gene|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|sr1] RNA and [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] mRNA to promote their base pairing and to control arginine metabolism [pubmed|31043113]
Protein family
CsrA/RsmA family (single member, according to UniProt)
[wiki|Domains]
Contacts FliW via its C-terminus (residues 49-58) [pubmed|27551070]
Structure
[PDB|1T3O]
[PDB|5DMB] ([protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[protein|93F328524989597C7D2329B25E665496C9631E87|csrA] complex) [Pubmed|27551070]
[AF|P33911]
Effectors of protein activity
[protein|93F328524989597C7D2329B25E665496C9631E87|csrA] is sequestered by interaction with [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW] (the two proteins form a conserved module in many bacteria) [Pubmed|21895793,27516547]
[wiki|Localization]
cytoplasm (according to UniProt)
Additional information
[protein|93F328524989597C7D2329B25E665496C9631E87|csrA] and [protein|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW] form a conserved module in many bacteria [Pubmed|21895793]
Expression and Regulation
Operons
genes
[gene|45F27C0789199626BA90D2C69E8F13525C911478|comFA]-[gene|D290247A43280532947B707F3AF388D7A7F404D3|comFB]-[gene|04D9D70C0BCFDCBD00B5156908C348135080A9C2|comFC]-[gene|049B1EBF64F0EBC04CE6D529C8EDB0B6B2184DF5|yvyF]-[gene|F03144BF8A187C8931938A21433431B8961E8EE7|flgM]-[gene|6F4CD36D4C1EE99EF7A503F44F53AEB8EFFEAB7F|flgN]-[gene|767ADBEF1B40CB3C74116CEA8CFB2FAF19C1FE7B|flgK]-[gene|82AB04023BCB57967C7501E6AEAC4BB593AA6480|flgL]-[gene|E5A59D2AF53FA220BA98D9653589A8F0D24F75B8|yviE]-[gene|6A6CBD75DCC33C804F853F47D37773930D398CFE|fliW]-[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
description
[Pubmed|22383849]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8412657], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|21736639], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|19898538], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8412657], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|8045879], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
genes
[gene|93F328524989597C7D2329B25E665496C9631E87|csrA]
description
[Pubmed|8969505]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|26577401], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
Biological materials
Mutant
MGNA-A392 (csrA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/392 NBRP B. subtilis, Japan]
GP469 (spc), available in [wiki|Jörg Stülke]'s lab
BKE35370 ([gene|93F328524989597C7D2329B25E665496C9631E87|csrA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
BKK35370 ([gene|93F328524989597C7D2329B25E665496C9631E87|csrA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCGTTTATTTTCCGCGATA, downstream forward: _UP4_TGAGGATTTTTTTATTTTTG
Expression vectors
pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [wiki|SPINE], in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
for expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844]: pGP383, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
pGP461 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-22 06:46:34
Author of last update: Jstuelk