gerBC
168
lipoprotein, nutrient receptor, germination response to the combination of glucose, fructose, and KCl
locus
BSU_35820
Molecular weight
42.31 kDa
pI
9.06
function
germination
product
part of the putative nutrient-gated [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[protein|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[protein|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC] ion channel
essential
no
synonyms
gerBC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5903 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,691,372 3,692,496
The protein
Catalyzed reaction/ biological activity
the [protein|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[protein|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[protein|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC] ion channel likely releases ions from the spore core in response to asparagine, glucose, fructose and K+ to trigger [category|SW.4.2.4|Germination], this requires the gerK locus [pubmed|37104613]
Protein family
[wiki|GerABKC lipoprotein family] (according to UniProt)
[wiki|Domains]
has an N-terminal signal sequence, lipidated on a Cys residue adjacent to the signal peptide [Pubmed|23335419]
Structure
[PDB|3N54] [Pubmed|20654628]
[AF|P39571]
Paralogous protein(s)
[protein|D937E26DF69D49B1341B37596AC6A8FBEEF8BA2E|gerAC]
[wiki|Localization]
outer surface of the inner spore membrane [Pubmed|23335419,21685283]
Additional information
700 molecules are present per spore [Pubmed|23749970]
Expression and Regulation
Operons
genes
[gene|3368743E6E03792DB83A38A19989123304DF7560|gerBA]-[gene|D70EC7C02BC79BED724489ABCB6356447F215B87|gerBB]-[gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]
description
[Pubmed|8012571]
regulation
expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|15699190,8012571]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|15699190,8012571], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
additional information
700 molecules are present per spore [PubMed|23749970]
Biological materials
Mutant
BKE35820 ([gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACGGAGAATTTTGATGCTG, downstream forward: _UP4_TAAGCAATCAAAAGGGTGCG
BKK35820 ([gene|93D4D6864686E6E559E6CEFA69BAC77EEFAA1BE3|gerBC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACGGAGAATTTTGATGCTG, downstream forward: _UP4_TAAGCAATCAAAAGGGTGCG
References
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Time of last update: 2024-10-10 11:47:16
Author of last update: Jstuelk