mtnE
168
glutamine transaminase
locus
BSU_13580
Molecular weight
43.88 kDa
pI
4.91
function
methionine salvage
product
glutamine transaminase
essential
no
synonyms
mtnE, ykrV
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,425,641 1,426,837
Phenotypes of a mutant
reduced growth with 5-methylthioribose as single sulfur source [Pubmed|24837359]
The protein
Catalyzed reaction/ biological activity
2-ketomethylthiobutyrate + glutamine -- 2-ketoglutaramate + methionine [Pubmed|24837359]
Protein family
[wiki|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP
Structure
[PDB|2O1B] (from Staphylococcus aureus, 42% identity)
[AF|O31665]
Paralogous protein(s)
[protein|99434727013FCCD6E570AE550560A8546F7F2A41|bacF]
Expression and Regulation
Operons
genes
[gene|938E08D2C50649A52EC06B9A08C6A73598E68CE1|mtnE]
description
[Pubmed|12022921]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12022921], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B321 (ykrV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1320 NBRP B. subtilis, Japan]
BKE13580 ([gene|938E08D2C50649A52EC06B9A08C6A73598E68CE1|mtnE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCTCACCTTCTAA, downstream forward: _UP4_TAACGATGGGATAATTCAAA
BKK13580 ([gene|938E08D2C50649A52EC06B9A08C6A73598E68CE1|mtnE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATTCTCACCTTCTAA, downstream forward: _UP4_TAACGATGGGATAATTCAAA
References
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Time of last update: 2025-04-04 23:51:33
Author of last update: Melvin.boenninger