sirB
168
sirohydrochlorin ferrochelatase
locus
BSU_15620
Molecular weight
28.69 kDa
pI
6.06
function
siroheme biosynthesis, sulfite reduction
product
sirohydrochlorin ferrochelatase
essential
no
synonyms
sirB, ylnE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2138 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,634,837 1,635,622
The protein
Catalyzed reaction/ biological activity
insertion of Fe2+ into the substrate sirohydrochlorin (SHC) in siroheme biosynthesis [pubmed|30778451]
2 H+ + siroheme --> Fe2+ + sirohydrochlorin (according to UniProt)
Protein family
CbiX family (single member, according to UniProt)
Structure
Apo-form: [PDB|5ZT8] [pubmed|30778451]
Co2+ bound: [PDB|5ZT7] ([pubmed|30778451]), [PDB|6JV6]
Ni2+ bound: [PDB|5ZT9]
Fe3+ bound: [PDB|5ZTA]
[AF|O34632]
Expression and Regulation
Operons
genes
[gene|51EB09C122A7166D662AE72842A44EC5FBA03134|cysH]-[gene|58A5A7F12664EC38AC21E24C1AD89671D7E648CF|cysP]-[gene|6CF2A9A517B641C44C0B46EF242BC5E668EA846B|sat]-[gene|3C71659E4868744A9301C26608EABF7B98217DCF|cysC]-[gene|92831D5A8E07A18BA6C6978123769D00D685BB45|ylnD]-[gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]-[gene|8BAB9B12D9D58A45446DC6CB7F48869089800FD3|ylnF]
description
[Pubmed|11004190]
regulation
induced by methionine starvation ([wiki|S-box]) [Pubmed|10094622]
the [wiki|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: transcription repression, [pubmed|], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
S-box: RNA switch, , the [wiki|S-box] [wiki|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|other_regulator:S-box|S-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11004190], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B369 (ylnE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1368 NBRP B. subtilis, Japan]
BKE15620 ([gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATAAAATTGCTTGTTTCA, downstream forward: _UP4_CAATTTGATTTTGATGGAGG
BKK15620 ([gene|9349C4646A56F933041EBE750F1BE86BAE7A60D9|sirB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATATAAAATTGCTTGTTTCA, downstream forward: _UP4_CAATTTGATTTTGATGGAGG
References
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Time of last update: 2024-11-03 11:22:44
Author of last update: Melvin.boenninger