folEB
168
GTP cyclohydrolase IB, required for the initial step of queuosine synthesis ([wiki|tRNA modification]), replaces [protein|7732A37D59E2643F232F2C0AFE51BCF7A79EDE29|folE] under conditions of zinc starvation
locus
BSU_03340
Molecular weight
34.63 kDa
pI
7.83
function
biosynthesis of folate and queuosine
product
zinc-independent GTP cyclohydrolase IB
essential
no
ec
3.5.4.16
synonyms
folEB, folE2, yciA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1469 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
364,259 → 365,173
The protein
Catalyzed reaction/ biological activity
GTP + H2O --> 7,8-dihydroneopterin 3'-triphosphate + formate + H+ (according to UniProt)
Protein family
GTP cyclohydrolase IV family (single member, according to UniProt)
[wiki|Cofactors]
activated by a variety of divalent cations [Pubmed|19767425]
Structure
[PDB|3D1T] [Pubmed|19767425]
[AF|P94398]
Expression and Regulation
Operons
genes
[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]-[gene|456AF184261976FAA73359E81443F7035F457D07|yciB]-[gene|F735802BB483C2C5437240AB2E9FC340EAA5B353|yczL]-[gene|75DB07A2704FEC2932BD0CDD24E2B3454E9551E1|zagA]
description
[Pubmed|9811636]
regulation
[wiki|folE2]: induced by zinc starvation ([protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]) [Pubmed|12426338]
regulatory mechanism
[protein|A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur]: repression, in the presence of zinc [Pubmed|12426338], in [regulon|protein:A0CD8CCB54AE9F10DE3C876FB47B9877C303862D|zur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12426338], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
BKK03340 (Δ[gene|93380E8EEB672C14C9BE2B5A6D8573FBCA149514|folEB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAGAAAACTCCTCTCA, downstream forward: _UP4_TTTTCGAAGGAAGTGGATAA
References
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Time of last update: 2025-03-15 03:27:20
Author of last update: Jstuelk