rsbP
168
protein serine phosphatase, energy PP2C, dephosphorylates [protein|AC64DA463250A090A62E50901EFE653C8F963872|rsbV], required for activation of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] during bacterial-fungal interaction
locus
BSU_34110
Molecular weight
45.87 kDa
pI
4.83
function
control of [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activity
product
protein serine phosphatase, energy PP2C
essential
no
ec
3.1.3.3
synonyms
rsbP, yvfP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2208 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,500,386 3,501,597
Phenotypes of a mutant
no induction of σB-dependent transcripts in response to carbon limitation
The protein
Catalyzed reaction/ biological activity
dephosphorylation of [protein|AC64DA463250A090A62E50901EFE653C8F963872|rsbV] in response to red light, this results in [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB] activation [Pubmed|19948797]
H2O + O-phospho-L(or D)-serine --> L(or D)-serine + phosphate (according to UniProt)
[wiki|Domains]
N-terminal [wiki|PAS domain], central coiled-coil domain
[wiki|PPM-type phosphatase domain] (aa 191-402) (according to UniProt)
Structure
[AF|O07014]
Effectors of protein activity
activity is stimulated upon interaction of the [protein|9284E58A2D5394A31658E70879874A1338CF531B|rsbP] oligomer with [wiki|RsbQ] [Pubmed|21980452]
Expression and Regulation
Operons
genes
[gene|9284E58A2D5394A31658E70879874A1338CF531B|rsbP]-[gene|53496D7AC05B20C5AEB6CB9B9B8825FC987B12C5|rsbQ]
description
[Pubmed|22383849]
Biological materials
Mutant
GP3323 (Δ[gene|9284E58A2D5394A31658E70879874A1338CF531B|rsbP]::''phleo''), available in [wiki|Jörg Stülke]'s lab
MGNA-A488 (yvfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/488 NBRP B. subtilis, Japan]
BKE34110 ([gene|9284E58A2D5394A31658E70879874A1338CF531B|rsbP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAGAGGGTCACCTCT, downstream forward: _UP4_TAAAATGATCCATGAGACAT
BKK34110 ([gene|9284E58A2D5394A31658E70879874A1338CF531B|rsbP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAAGAGGGTCACCTCT, downstream forward: _UP4_TAAAATGATCCATGAGACAT
Expression vectors
pGP3599: expression of [gene|9284E58A2D5394A31658E70879874A1338CF531B|rsbP] by [wiki|pBQ200] in B. subtilis, available in [wiki|Jörg Stülke]'s lab
lacZ fusion
GP3328 (based on [wiki|pAC5]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Chet Price], Davis, USA [http://foodscience.ucdavis.edu/price_lab/pricelab.html homepage]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 11:49:54
Author of last update: Jstuelk