gpr
168
germination protease (degradation of SASPs)
locus
BSU_25540
Molecular weight
40.12 kDa
pI
5.2
function
degradation of SASPs
product
germination protease (degradation of SASPs)
essential
no
synonyms
gpr
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5911 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,634,505 2,635,611
The protein
Catalyzed reaction/ biological activity
degradation of small, acid-soluble spore proteins (SASPs) [Pubmed|8478323]
Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala (according to UniProt)
Protein family
peptidase A25 family (single member, according to UniProt)
Structure
[PDB|1C8B] (from B. megaterium, 68% identity) [pubmed|10864493]
[AF|P22322]
[wiki|Localization]
inner spore membrane [Pubmed|26731423]
Expression and Regulation
Operons
genes
[gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]-[gene|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP]
description
[Pubmed|16497325]
regulation
expressed during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF], [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], [wiki|SpoVT]) [Pubmed|16497325,1840582]
regulatory mechanism
[protein|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]: repression, [Pubmed|16497325], in [regulon|protein:01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT regulon]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|16497325,1840582], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|1840582], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
BKE25540 ([gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
BKK25540 ([gene|91C5AF928EB8E00F12D1BC0E788E1CB116F87B04|gpr]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAGGGGCCTCCTTCAC, downstream forward: _UP4_TAAACAGTTCTACTTCCTCT
References
Page visits: 3588
Time of last update: 2024-10-15 05:23:07
Author of last update: Jstuelk