scpB

scpB
168

part of the [wiki|condensin] complex, chromosomal origin condensation and segregation

locus
BSU_23210
Molecular weight
21.89 kDa
pI
4.25
Protein length
197 aaSequenceBlast
Gene length
594 bpSequenceBlast
function
segregation of replication origins
product
DNA segregation and condensation protein
essential
no
synonyms
scpB, ypuH, spcB
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1386 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,425,248  2,425,841
Phenotypes of a mutant
essential [Pubmed|24440399], non-essential according to [Pubmed|28189581]
''[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
The protein
Catalyzed reaction/ biological activity
negatively regulates binding of the [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] NTD to the [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] neck region [pubmed|28286005]
Protein family
scpB family (single member, according to UniProt)
Structure
[PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] (aa 1-183) [Pubmed|23353789]
[AF|P35155]
[wiki|Localization]
nucleoid (Multiple) [Pubmed|16479537]
the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]
Expression and Regulation
Operons
genes
[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
description
[Pubmed|7934830]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
Open in new tab

[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]→[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]

2024-10-15 03:16:50

ghost

119

962f5d9cd284750c7b8d6dafae170ff7097452f9

E653E2EC69211554D00603C6268E7EDE01F77C4F

Biological materials
Mutant
MGNA-A105 (ypuH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/105 NBRP B. subtilis, Japan]
BKE23210 ([gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATAGCTTTCCAATTCA,  downstream forward: _UP4_TAGAAGTTTAATGAATCGCG
BKK23210 ([gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATAGCTTTCCAATTCA,  downstream forward: _UP4_TAGAAGTTTAATGAATCGCG
References
Reviews
22933559,22934648,24118085,25460803,26706151,29182912,27075410,34220773
Original Publications
12100548,12065423,12421306,22385855,16479537,19450516,12897137,7934830,11948165,23353789,24440399,24440393,25071173,25557547,25951515,26253537,23475963,26295962,26725510,26904953,28189581,28154080,28286005,28689660,30192981,31201090,31175837,32250245,33472056,33910021,34312537,34346312,35474142

9137D162E6331E2B127472BCF5F49A9AE46266F3

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Time of last update: 2024-10-14 04:27:22

Author of last update: Jstuelk