tagB
168
putative CDP-glycerol:glycerol phosphate glycerophosphotransferase
locus
BSU_35760
Molecular weight
44.40 kDa
pI
8.93
function
biosynthesis of teichoic acid
product
putative CDP-glycerol:glycerol phosphate glycerophosphotransferase
essential
yes
ec
2.7.8.-
synonyms
tagB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1887 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,682,173 3,683,318
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
adds a single glycerol phosphate residue to the non-reducing end of the undecaprenol-phosphate-linked N-acetylmannosamine-(1,4)-N-acetylglucosamine-1-phosphate [Pubmed|17660278]
CDP-glycerol + N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate --> 4-O-[(2R)-glycerylphospho]-N-acetyl-β-D-mannosaminyl-(1→4)-N-acetyl-α-D-glucosaminyl di-trans,octa-cis-undecaprenyl diphosphate + CMP + H+ (according to UniProt)
Protein family
CDP-glycerol glycerophosphotransferase family (with [protein|E730E41546298960A4B907FCB7F36C495AA9602B|tagF], according to UniProt)
Structure
[PDB|3L7I] (from Staphylococcus epidermidis, corresponds to aa 113 ... 380, 31% identity) [pubmed|20400947]
[AF|P27621]
[wiki|Localization]
cell membrane [Pubmed|17660278]
Expression and Regulation
Operons
genes
[gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA]-[gene|911A4D957A6C0DFC636A152395119DBFC777DDA2|tagB]
description
[Pubmed|7952180]
regulation
repressed when phosphate is available ([protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]) [Pubmed|9457886]
the leader mRNA is processed upstream of [gene|72A89412C53703F96F835D26307263F5D1A4AB4E|tagA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: repression, [Pubmed|9457886], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
[protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR]: activation, [Pubmed|12950927], in [regulon|protein:7F340423A34CE40D1F1AA8D373F7C4B859A6496D|walR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7581998], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
References
Reviews
Original Publications
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Time of last update: 2024-12-19 19:14:19
Author of last update: Melvin.boenninger