parC
168
subunit of DNA topoisomerase IV
locus
BSU_18100
Molecular weight
91.14 kDa
pI
6.31
function
chromosome segregation and compaction, resolution of head-on [category|SW.3.1.1|DNA replication]-[category|SW.3.2.1|Transcription] conflicts
product
subunit of DNA topoisomerase IV
essential
yes
ec
5.99.1.-
synonyms
parC, grlA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0188 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,935,448 1,937,868
Phenotypes of a mutant
essential [Pubmed|12682299]
a ''parC'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]
upon overexpression, topoisomerase IV ([protein|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]-[protein|9075DD0CC0B8554F0379732453665F43C70EFC26|parC]) can take over the functions of the type 1A topoisomerases [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA] and [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|topB] [pubmed|30957856]
The protein
Catalyzed reaction/ biological activity
ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
binds to abasic DNA lesions [pubmed|37250769]
Protein family
Topoisomerase IIA [protein|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]/[protein|9075DD0CC0B8554F0379732453665F43C70EFC26|parC] subunit family (together with [protein|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]) (according to UniProt)
Structure
[PDB|4I3H] (the [protein|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]-[protein|9075DD0CC0B8554F0379732453665F43C70EFC26|parC] complex from ''Streptococcus pneumoniae'', 63% identity) [Pubmed|23965305]
[PDB|1WP5] (C-terminal domain, Geobacillus stearothermophilus)
[AF|Q45066]
Paralogous protein(s)
[protein|B207C7328177924B16F1C606F667BEADF12CA069|gyrA]
[wiki|Localization]
cytoplasm (Homogeneous) [Pubmed|16479537]
polar localization in actively growing cells [Pubmed|9539793]
Expression and Regulation
Operons
genes
[gene|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]-[gene|9075DD0CC0B8554F0379732453665F43C70EFC26|parC]
description
[Pubmed|16267290]
regulation
induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|16267290]
regulatory mechanism
[protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|30957856], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''parE'' [Pubmed|27120414]
References
Reviews
Original Publications
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Time of last update: 2024-12-21 20:22:30
Author of last update: Jstuelk