plsX
168
acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
locus
BSU_15890
Molecular weight
35.61 kDa
pI
5.49
function
biosynthesis of phospholipids
product
acyl-acyl carrier protein (ACP):phosphate acyltransferase
essential
yes
ec
2.3.1.n2
synonyms
plsX, ylpD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0416 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,662,547 1,663,548
Phenotypes of a mutant
essential [Pubmed|24224907,12682299]
depletion results in sporulation deficiency (via [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|spoIIR]) [Pubmed|22111992]
a ts mutant forms an abberant Z-ring and has a [wiki|cell division] defect [Pubmed|24224907]
depletion results in accumulation of malonyl-CoA and of long-chain-ACPs [pubmed|32671874]
The protein
Catalyzed reaction/ biological activity
catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
fatty acyl-[ACP] + phosphate --> acyl phosphate + holo-[ACP] (according to UniProt)
Protein family
plsX family (single member, according to UniProt)
Structure
[PDB|1VI1] [Pubmed|16021622]
[PDB|6A1K] [pubmed|31453380]
[AF|P71018]
[wiki|Localization]
localizes to regions of increased fluidity in the membrane (via a short amphipathic α-peptide) [pubmed|31453380,31925177]
uniformly distributed on the membrane of most cells, but occasionally appears as membrane foci as well [Pubmed|26816052]
in the presence of daptomycin, PlsX delocalizes from the membrane to the cytoplasm [Pubmed|27791134]
Expression and Regulation
Operons
genes
[gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]-[gene|8F5612395EBF90BEF4C80056EECAFC572384E7F8|plsX]-[gene|BB8542A6444564B21FF5DB6E2C75192C6DA2DFB8|fabD]-[gene|439B468A13137000FB42E9389391CB4986FFED84|fabG]-[gene|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|acpA]
description
[Pubmed|19850612]
regulation
''[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]'': repressed in the absence of malonyl-CoA or malonyl-[protein|8D260693D0C69442BCB9F3D8480E8425ACFE34D2|ACP] ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [Pubmed|12737802]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]: repression, [Pubmed|12737802], in [regulon|protein:FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR regulon]
[protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]: activation, ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]) [Pubmed|16091051], in [regulon|protein:7832489F11F606BCF637EC23BAAB41AD10237EBB|comA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16932747], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in Matsumoto lab [Pubmed|19282621]
References
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Time of last update: 2024-12-21 05:39:26
Author of last update: Jstuelk