flhO

flhO
168

flagellar basal-body rod protein, required for hook assembly

locus
BSU_36400
Molecular weight
29.28 kDa
pI
4.73
Protein length
Gene length
function
motility and chemotaxis
product
flagellar basal-body rod protein
essential
no
synonyms
flhO, flgG, yvyA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG4786 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,746,279  3,747,091
Phenotypes of a mutant
lack of [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-dependent gene expression (no ''[gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]'' expression), no motility  [Pubmed|22730131]
defective in [category|SW.4.1.4|Swarming] and flagellar assembly [pubmed|30201778,35638827]
The protein
Catalyzed reaction/ biological activity
assembly of the flagellar hook [Pubmed|22730131]
part of the flagellar rod-hook (in [protein|7E8F88F7A9CAD4ED0927CDC6AAFDEDC08197C709|fliE]-[protein|1C6C8DB02E10EAC0588389D9F0D3AF3E19172C5C|flgB]-[protein|CC423DE6481353699B0CD3A2B8BBC36967A832CC|flgC]-[protein|8EFE091CFB89FC406CD60776977C9C302C7826E0|flhO]-[protein|13F35BC7EE8215D7A92A639BEB18647AC276D215|flhP]-[protein|DC906ED8D787602B5796BAD8FD81F02C4BAC8D13|flgE] out, connection to [protein|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag]) [pubmed|39211283]
Protein family
[wiki|Flagella basal body rod proteins family] (according to UniProt)
Structure
[PDB|5JXL] (from Campylobacter jejuni, 30% identity) [pubmed|27811912]
[AF|P39752]
Paralogous protein(s)
[protein|DC906ED8D787602B5796BAD8FD81F02C4BAC8D13|flgE], [protein|13F35BC7EE8215D7A92A639BEB18647AC276D215|flhP]
[wiki|Localization]
extracellular (no signal peptide) [Pubmed|18957862]
flagellum hook (according to UniProt)
Expression and Regulation
Operons
genes
[gene|8EFE091CFB89FC406CD60776977C9C302C7826E0|flhO]-[gene|13F35BC7EE8215D7A92A639BEB18647AC276D215|flhP]
description
[Pubmed|15033535,22730131]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [Pubmed|15033535,22730131], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
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[gene|8EFE091CFB89FC406CD60776977C9C302C7826E0|flhO]→[gene|13F35BC7EE8215D7A92A639BEB18647AC276D215|flhP]

2024-10-07 00:04:11

ghost

85

385b168442c3303a06fdd6bd502f1aa8ac4ee20f

E7BD43BA202568F81E22897F4FC7365908069813

Biological materials
Mutant
BKE36400 ([gene|8EFE091CFB89FC406CD60776977C9C302C7826E0|flhO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATTTCCCACCTCTAAAA,  downstream forward: _UP4_TAAGAAAACGGAAGAAAAAA
BKK36400 ([gene|8EFE091CFB89FC406CD60776977C9C302C7826E0|flhO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36400 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAATTTCCCACCTCTAAAA,  downstream forward: _UP4_TAAGAAAACGGAAGAAAAAA
References
Reviews
26490009
Original Publications
18957862,9023218,7836311,15033535,30201778,27811912,35638827,36815589

8EFE091CFB89FC406CD60776977C9C302C7826E0

Page visits: 2550

Time of last update: 2024-10-09 07:42:25

Author of last update: Jstuelk