glcK
168
glucose kinase (D-glucose:ATP)
locus
BSU_24850
Molecular weight
33.39 kDa
pI
5.99
function
phosphorylation of the free glucose moiety resulting from cleavage of di-and oligosaccharides
product
glucose kinase (D-glucose:ATP)
essential
no
ec
2.7.1.2
synonyms
glcK, yqgR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1940 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,570,606 2,571,571
The protein
Catalyzed reaction/ biological activity
ATP + D-glucose --> ADP + D-glucose 6-phosphate + H+ (according to UniProt)
Protein family
ROK (NagC/XylR) family (with [protein|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE] and [protein|AF4395D134485290F4AA307B48494FED39E52CD7|xylR], according to UniProt)
Structure
[PDB|2QM1] (from ''Enterococcus faecalis'', 38% identity, 52% similarity)
[AF|P54495]
Paralogous protein(s)
[protein|AF4395D134485290F4AA307B48494FED39E52CD7|xylR]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|89C51789FBB2EE86771ABD8629FD99748ED5D4EF|yqgP]-[gene|C44F6723A2F1A627A0B9042871A07F5365D6121A|yqgO]-[gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]-[gene|2B33F2F9C2FB0767B72A29D060037393C88ACEE5|yqgS]
description
[pubmed|22383849]
additional information
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]∼P binds to the promoter region, but regulation has not been explored [pubmed|25666134]
Biological materials
Mutant
GP2 (spc), available in [wiki|Jörg Stülke]'s lab
BKE24850 ([gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT
BKK24850 ([gene|8E10A8B566BC25CBDDEF1503656A4234FD29ACB8|glcK]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24850 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCATATCTCGTCCATTTTTA, downstream forward: _UP4_TAAAATTGTGTAAATGAAAT
References
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Time of last update: 2024-12-30 12:04:13
Author of last update: Melvin.boenninger