ssbA
168
single-strand DNA-binding protein, part of the [wiki|replisome], promoter of [protein|search|RecA ]DNA repair center assembly, required to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU_40900
Molecular weight
18.60 kDa
pI
4.82
function
[category|SW.3.1.1|DNA replication], [category|SW.3.1.5|DNA repair/ recombination]
product
single-strand DNA-binding protein
essential
yes
synonyms
ssbA, ssb
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0629 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,198,886 4,199,404
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
SsbA inhibits ssDNA phosphorylase activity of [protein|64C6D783FF3F41C81B216F798A1DC8071345B1ED|pnpA] [Pubmed|21859751]
SsbA co-assembles onto [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]-coated ssDNA [Pubmed|22373918]
important for [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] DNA repair center assembly by recruiting [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] and [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|recR] to the [wiki|replisome] [Pubmed|24891441]
[protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] as an accessory factor [Pubmed|25138221]
inhibits stable [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleation and polymerization on ssDNA [pubmed|28911099]
the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] onto any fragmented linear SPP1 ssDNA[pubmed|31876108]
[wiki|Domains]
SSB domain (aa 1-104) (according to UniProt)
Structure
[PDB|6BHX] (complex with ssDNA) [Pubmed|30472092]
[PDB|6BHW] [Pubmed|30472092]
[AF|P37455]
Modification
phosphorylated on Arg-76 [Pubmed|22517742]
phosphorylated on Tyr-82 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ] [Pubmed|16549871]
Effectors of protein activity
Phosphorylation of Ssb increases binding to single-stranded DNA in vitro almost 200-fold [Pubmed|16549871]
Paralogous protein(s)
[protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]
[wiki|Localization]
forms foci [Pubmed|21958350]
Nucleoid (Mid-cell) [Pubmed|16479537]
Expression and Regulation
Operons
genes
[gene|BD162A17E5F3F9AB49E478CE72D0FE92D82E6673|yyzM]-[gene|FEE1952DFA3D6A5E2DC108B3B203B526642E2C68|yyaE]-[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
description
[Pubmed|22383849]
genes
[gene|C0F5FAD6DF89DA9E0FFC8F888D7CED6AA42D9C73|yyaF]-[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
description
[Pubmed|14762004]
regulation
[protein|search|ComK]: transcription activation [Pubmed|11948146]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|24310371], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|9D25B8F4579C41E77AE9CDD18CAD6DA37F3DD0A2|rpsF]-[gene|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[gene|C0B926F1134A7A3FEEC886F9AF67F694C8E0C089|rpsR]
description
[Pubmed|24310371,22383849]
regulation
[protein|search|ComK]: transcription activation [Pubmed|11948146]
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
Page visits: 5582
Time of last update: 2024-12-18 08:32:41
Author of last update: Jstuelk