scpA
168
part of the [wiki|condensin] complex, chromosomal origin condensation and segregation
locus
BSU_23220
Molecular weight
29.46 kDa
pI
4.79
function
segregation of replication origins
product
DNA segregation and condensation protein
essential
yes
synonyms
scpA, ypuG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1354 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,425,831 2,426,586
Phenotypes of a mutant
essential [Pubmed|12682299]
''[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
The protein
Protein family
scpA family (single member, according to UniProt)
Structure
[PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]) [Pubmed|23353789]
[PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] (aa 1-183) [Pubmed|23353789]
[AF|P35154]
[wiki|Localization]
nucleoid (Multiple) [Pubmed|16479537]
the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] [Pubmed|24440393,23475963]
the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]
Expression and Regulation
Operons
genes
[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
description
[Pubmed|7934830]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
Biological materials
Mutant
MGNA-A104 (ypuG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/104 NBRP B. subtilis, Japan]
References
Reviews
Original Publications
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Time of last update: 2024-12-30 18:06:56
Author of last update: Jstuelk