scpA

scpA
168

part of the [wiki|condensin] complex, chromosomal origin condensation and segregation

locus
BSU_23220
Molecular weight
29.46 kDa
pI
4.79
Protein length
Gene length
function
segregation of replication origins
product
DNA segregation and condensation protein
essential
yes
synonyms
scpA, ypuG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1354 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,425,831  2,426,586
Phenotypes of a mutant
essential [Pubmed|12682299]
''[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]
The protein
Protein family
scpA family (single member, according to UniProt)
Structure
[PDB|3ZGX] (complex between the head domain of [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]) [Pubmed|23353789]
[PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] (aa 1-183) [Pubmed|23353789]
[AF|P35154]
[wiki|Localization]
nucleoid (Multiple) [Pubmed|16479537]
the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|parA] [Pubmed|24440393,23475963]
the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]
Expression and Regulation
Operons
genes
[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]-[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]
description
[Pubmed|7934830]
regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
regulatory mechanism
stringent response: negative regulation, in [regulon|other_regulator:stringent response|stringent response]
Open in new tab

[gene|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]→[gene|3CC628BCF8F9F45E598060B775008B361DB2127A|ypuI]

2024-10-15 03:16:50

ghost

119

962f5d9cd284750c7b8d6dafae170ff7097452f9

E653E2EC69211554D00603C6268E7EDE01F77C4F

Biological materials
Mutant
MGNA-A104 (ypuG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/104 NBRP B. subtilis, Japan]
References
Reviews
22934648,22933559,24118085,25460803,26706151,29182912,27075410,34220773
Original Publications
12100548,12421306,12065423,7934830,16479537,19450516,15009890,11948165,23353789,23475963,22385855,24440399,24440393,25557547,26253537,25951515,26295962,26725510,26904953,28154080,28286005,28689660,30192981,31201090,31175837,32250245,33472056,33910021,34312537,34346312,35474142

8DD968C97FDC0E81293887E2FCB7EE1A9416A333

Page visits: 4510

Time of last update: 2024-10-16 02:47:10

Author of last update: Jstuelk