ilvD
168
dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate)
locus
BSU_21870
Molecular weight
59.38 kDa
pI
5.32
function
biosynthesis of branched-chain amino acids
product
dihydroxy-acid dehydratase (2,3-dihydroxy-3-methylbutanoate, 2,3-dihydroxy-3-methylpentanoate)
essential
no
ec
4.2.1.9
synonyms
ilvD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0129 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,300,762 2,302,438
The protein
Catalyzed reaction/ biological activity
2,3-dihydroxy-3-methylbutanoate --> 3-methyl-2-oxobutanoate + H2O(according to UniProt)
Protein family
IlvD/Edd family (single member, according to UniProt)
[wiki|Cofactors]
Fe-S cluster [pubmed|29292548]
Structure
[PDB|2GP4] (apo form of 6-phosphogluconate dehydratase from ''Shewanella oneidensis'', 33% identity)
[AF|P51785]
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|8D08E5F1EC57A9B3EFFCD7D12D8F16B3B28AEFF0|ilvD]
description
[Pubmed|15060025]
regulation
repressed by casamino acids [Pubmed|12107147]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455] [pubmed|18083814], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
Biological materials
Mutant
BKE21870 ([gene|8D08E5F1EC57A9B3EFFCD7D12D8F16B3B28AEFF0|ilvD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTGATCCTCCTAAATT, downstream forward: _UP4_TAGATAGCTGGGGACACTTT
BKK21870 ([gene|8D08E5F1EC57A9B3EFFCD7D12D8F16B3B28AEFF0|ilvD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTGATCCTCCTAAATT, downstream forward: _UP4_TAGATAGCTGGGGACACTTT
lacZ fusion
pGP522 (in [wiki|pAC5]), pGP235 (in [wiki|pAC5]), both available in [wiki|Jrg Stlke]'s lab
a series of promoter deletions in [wiki|pAC6] is available in [wiki|Jrg Stlke]'s lab
References
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Time of last update: 2024-10-08 10:01:57
Author of last update: Melvin.boenninger