yacO
168
putative 23S rRNA 2'-O-ribose G2251 methyltransferase
locus
BSU_00960
Molecular weight
27.36 kDa
pI
8.63
function
rRNA modification
product
putative 23S rRNA methyltransferase
essential
no
ec
2.1.1.-
synonyms
yacO, rlmB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0566 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
115,269 116,018
The protein
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
class IV-like SAM-binding methyltransferase superfamily (with [protein|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR] and [protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP], according to UniProt)
[wiki|Cofactors]
SAM (according to UniProt)
Structure
[PDB|1GZ0] (from ''E. coli'', 44% identity) [Pubmed|12377117]
[AF|Q06753]
Paralogous protein(s)
[protein|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|132E91DB8C81CD8C4F5160B2C068B74DA10AE224|gltX]-[gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE]-[gene|E5826A99769E2FE45959A9D623D6467A8DDDEB81|cysS]-[gene|031FDF8443A8A4D5B042B7591EBE2E6C74F07702|mrnC]-[gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]-[gene|172652156ABFE72D5BAAC22729CE17CA947538B5|yacP]
description
[Pubmed|7510287]
regulation
expression transiently increases in the forespore [Pubmed|22848659]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, overlaps a transcription terminator upstream of [gene|5B9D5DA130DC3654F386684156BDC350DD05DB60|cysE], in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7510287], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B884 (yacO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1883 NBRP B. subtilis, Japan]
BKE00960 ([gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGCATTTTTCCCTATGA, downstream forward: _UP4_AACCCTGTGGGAGAATAAAG
BKK00960 ([gene|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00960 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGCATTTTTCCCTATGA, downstream forward: _UP4_AACCCTGTGGGAGAATAAAG
References
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Time of last update: 2025-01-14 03:56:37
Author of last update: Jstuelk