pucH
168
allantoinase
locus
BSU_32410
Molecular weight
48.17 kDa
pI
5.06
function
purine utilization
product
allantoinase
essential
no
ec
3.5.2.5
synonyms
pucH, yunH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0044 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,327,247 3,328,587
The protein
Catalyzed reaction/ biological activity
[metabolite|allantoin] + H2O --> [metabolite|allantoate] + H+ (according to UniProt)
Protein family
[wiki|Metallo-dependent hydrolases superfamily] (according to UniProt)
Structure
[PDB|3HM7] (from ''Bacillus halodurans'', 44% identity, 65% similarity)
[AF|O32137]
Paralogous protein(s)
[protein|CA16FD3507DCCADB1E01DF25B42C3405C9205B05|pyrC]
Expression and Regulation
Operons
genes
[gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]
description
[Pubmed|12029039]
regulation
induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|pucR]: activation, [Pubmed|12029039], in [regulon|protein:52C1601482C26400A524E880334BB801F832D6ED|pucR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|12029039], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A933 (yunH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/933 NBRP B. subtilis, Japan]
BKE32410 ([gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCCCCCCATGTC, downstream forward: _UP4_TAAGCCCGGCGGATTTTACC
BKK32410 ([gene|8C53FEBB2BCCE45A686119B7ECE4C266D2C0822A|pucH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGATCCCCCCATGTC, downstream forward: _UP4_TAAGCCCGGCGGATTTTACC
References
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Time of last update: 2024-10-11 21:01:03
Author of last update: Jstuelk