alsR
168
transcriptional activator of the [gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD] operon
locus
BSU_36020
Molecular weight
34.18 kDa
pI
7.29
function
regulation of acetoin synthesis ([gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD])
product
transcriptional activator ([wiki|LysR family])
essential
no
synonyms
alsR
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,711,498 3,712,406
Phenotypes of a mutant
reduced acetoin production [pubmed|31113899]
impaired biofilm development [pubmed|31113899]
The protein
Catalyzed reaction/ biological activity
binds the promoter region of the [gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD] operon in the presence of acetate, activates transcription [pubmed|30039521]
Protein family
[wiki|LysR family] (according to UniProt)
[wiki|Domains]
[wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Structure
[PDB|2H98] (from Acinetobacter baylyi, 34% identity)
[AF|Q04778]
Effectors of protein activity
acetate (2 molecules per molecule of AlsR) acts as co-factor that triggers activity [pubmed|30039521]
Expression and Regulation
Operons
genes
[gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]-[gene|EB8E3F88BC1ED2BAD5A98D609EDAA002261150A5|ywrK]
description
[Pubmed|22383849]
Biological materials
Mutant
AMBs3 (spc), available in [wiki|Jörg Stülke]'s lab
1A978 ( ''alsR''::''cat''), [Pubmed|7685336], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A978&Search=1A978 BGSC]
BKE36020 ([gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATATGCATTCCTTT, downstream forward: _UP4_TGAACAATACTTGCAATCTC
BKK36020 ([gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCAATATGCATTCCTTT, downstream forward: _UP4_TGAACAATACTTGCAATCTC
labs
[wiki|Elisabeth Härtig], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]
References
Reviews
Original Publications
Page visits: 5023
Time of last update: 2024-12-05 09:10:39
Author of last update: Melvin.boenninger