alsR

alsR
168

transcriptional activator of the [gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD] operon

locus
BSU_36020
Molecular weight
34.18 kDa
pI
7.29
Protein length
Gene length
function
regulation of acetoin synthesis ([gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD])
product
transcriptional activator ([wiki|LysR family])
essential
no
synonyms
alsR

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,711,498  3,712,406
Phenotypes of a mutant
reduced acetoin production [pubmed|31113899]
impaired biofilm development [pubmed|31113899]
The protein
Catalyzed reaction/ biological activity
binds the promoter region of the [gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD] operon in the presence of acetate, activates transcription [pubmed|30039521]
Protein family
[wiki|LysR family] (according to UniProt)
[wiki|Domains]
[wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Structure
[PDB|2H98] (from Acinetobacter baylyi, 34% identity)
[AF|Q04778]
Effectors of protein activity
acetate (2 molecules per molecule of AlsR) acts as co-factor that triggers activity [pubmed|30039521]
Expression and Regulation
Operons
genes
[gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]-[gene|EB8E3F88BC1ED2BAD5A98D609EDAA002261150A5|ywrK]
description
[Pubmed|22383849]
Open in new tab

[gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]→[gene|EB8E3F88BC1ED2BAD5A98D609EDAA002261150A5|ywrK]

2024-10-14 04:01:18

Jstuelk

59

0c4c9296f621b434a75faa015427e4c02d6b9b1d

AA138F6CC87F971586EBC682344D0BD8D693627E

Biological materials
Mutant
AMBs3 (spc), available in [wiki|Jörg Stülke]'s lab
1A978 ( ''alsR''::''cat''), [Pubmed|7685336], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A978&Search=1A978 BGSC]
BKE36020 ([gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAATATGCATTCCTTT,  downstream forward: _UP4_TGAACAATACTTGCAATCTC
BKK36020 ([gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAATATGCATTCCTTT,  downstream forward: _UP4_TGAACAATACTTGCAATCTC
labs
[wiki|Elisabeth Härtig], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]
References
Reviews
23046954
Original Publications
7685336,21821766,22344650,23576037,22178965,23695583,23836140,30039521,31113899

8BA7714236EBFDBB9987F1DACC9775AD974C743E

Page visits: 4894

Time of last update: 2024-10-14 11:18:09

Author of last update: Melvin.boenninger