spoIIIAF

spoIIIAF
168

component of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] type III secretion system residing in the forespore membrane, required for [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activation

locus
BSU_24380
Molecular weight
23.14 kDa
pI
4.95
Protein length
Gene length
function
activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]
product
component of a type III secretion system
essential
no
synonyms
spoIIIAF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5827 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,533,692  2,534,312
The protein
Structure
[PDB|6DCS] [pubmed|29886194]
[AF|P49783]
[wiki|Localization]
membrane protein [Pubmed|18485064]
Expression and Regulation
Operons
genes
[gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]-[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|1766372]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,18485064], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Open in new tab

[gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]→[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]

2024-11-30 23:16:02

Jstuelk

73

2962C722B0487E202C1BFF79DD1ECDECA2A12F2C

1576F70579A1A6BA3390B10D16041D0C1E75C377

genes
[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|18485064]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
Open in new tab

[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]→[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]

2024-11-05 11:13:02

Jstuelk

90

8E4AE6460669048935F7AFEA330AB034477EDE0F

D37B011018A3858E66098C2BA5678469F573873A

Biological materials
Mutant
BKE24380 ([gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24380 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAAAAAACTCATTTGCCTGC,  downstream forward: _UP4_ATGGAAGGCGGGGAGAGTGT
BKK24380 ([gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24380 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TAAAAAACTCATTTGCCTGC,  downstream forward: _UP4_ATGGAAGGCGGGGAGAGTGT
References
Reviews
31350897,33651833,35638784
Original Publications
1766372,18485064,19609349,29886194

8A8C67AB955660FA41039B45F60A5B9F7B57704E

Page visits: 2011

Time of last update: 2024-12-03 01:55:16

Author of last update: Jstuelk