rlmP
168
SPOUT methyltransferase, forms 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA
locus
BSU_28650
Molecular weight
26.75 kDa
pI
5.18
function
formation of 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA
product
SPOUT methyltransferase
essential
no
ec
2.1.1.-
synonyms
ysgA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0566 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,930,827 2,931,573
Phenotypes of a mutant
essential [Pubmed|17114254], non-essential according to [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
forms 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA [pubmed|35710145]
modifies free 23S rRNA but not the fully assembled ribosome nor the 50S subunit, suggesting that the modification occurs early during ribosome biogenesis [pubmed|35710145]
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
class IV-like SAM-binding methyltransferase superfamily (with [protein|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO] and [protein|03BA7AEE17739689D632A35D9C3329EA0982C98A|cspR], according to UniProt)
[wiki|Cofactors]
SAM (according to UniProt)
Structure
[PDB|4X3M]
[AF|P94538]
Paralogous protein(s)
[protein|8CB595F84D00D1262EB13E6CF47BE6F00EDE16A5|yacO]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Biological materials
Mutant
MGNA-B006 (ysgA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1005 NBRP B. subtilis, Japan]
BKE28650 ([gene|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTCTATGTGGCGCTCC, downstream forward: _UP4_TAGTGTTGCGCCAAGCGTTG
BKK28650 ([gene|8A2898B80D0FFA56C5AA86C6F09D886A62AABBDA|rlmP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGTCTATGTGGCGCTCC, downstream forward: _UP4_TAGTGTTGCGCCAAGCGTTG
References
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Time of last update: 2024-10-03 09:47:09
Author of last update: Jstuelk