pdaA
168
N-acetylmuramic acid deacetylase, spore cortex peptidoglycan synthesis
locus
BSU_07980
Molecular weight
29.92 kDa
pI
7.32
function
spore cortex peptidoglycan synthesis
product
N-acetylmuramic acid deacetylase
essential
no
synonyms
pdaA, yfjS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0726 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
869,559 870,350
Phenotypes of a mutant
improved UV resistance of spores at the cost of attenuated heat resistance and viable spore yield [pubmed|38925118]
The protein
Catalyzed reaction/ biological activity
de-N-acetylation of N-acetylglucosamine and N-acetyl-muramic acid for spore cortex peptidoglycan [Pubmed|15687192]
Protein family
[wiki|polysaccharide deacetylase family] (according to UniProt)
[wiki|Domains]
[wiki|NodB homology domain] (aa 66-247) (according to UniProt)
Structure
[PDB|1W17] [pubmed|15251431]
[AF|O34928]
Expression and Regulation
Operons
genes
[gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]
description
[Pubmed|12374835]
regulation
expressed late during sporulation in the forespore ([protein|search|SigG]) [Pubmed|12374835,15699190]
sigma factors
[protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]: sigma factor, [Pubmed|12374835,15699190], in [regulon|protein:5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG regulon]
Biological materials
Mutant
MGNA-C273 (yfjS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2271 NBRP B. subtilis, Japan]
BKE07980 ([gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG, downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC
BKK07980 ([gene|88E167795BBCB8529E322E3A4DC9965EA6AAA617|pdaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07980 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGCCAGCGCTCCTTCTG, downstream forward: _UP4_TAAAAGAGAAAGACCTCTCC
References
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Time of last update: 2024-12-21 15:05:15
Author of last update: Jstuelk